NF-κBp50 and HDAC1 Interaction Is Implicated in the Host Tolerance to Infection Mediated by the Bacterial Quorum Sensing Signal 2-Aminoacetophenone

• Published in: Frontiers in microbiology Vol. 8; p. 1211
• Main Authors: Bandyopadhaya, Arunava, Tsurumi, Amy, Rahme, Laurence G
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 30-06-2017
• Subjects: 2-aminoacetophenone; CBP/p300; HDAC1; inflammatory cytokines; Microbiology; NF-κB; quorum sensing; NF-κB; 2-aminoacetophenone; quorum sensing; inflammatory cytokines; HDAC1; CBP/p300
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2017.01211

Some bacterial quorum sensing (QS) small molecules are important mediators of inter-kingdom signaling and impact host immunity. The QS regulated small volatile molecule 2-aminoacetophenone (2-AA), which has been proposed as a biomarker of
colonization in chronically infected human tissues, is critically involved in "host tolerance training" that involves a distinct molecular mechanism of host chromatin regulation through histone deacetylase (HDAC)1. 2-AA's epigenetic reprogramming action enables host tolerance to high bacterial burden and permits long-term presence of
without compromising host survival. Here, to further elucidate the molecular mechanisms of 2-AA-mediated host tolerance/resilience we investigated the connection between histone acetylation status and nuclear factor (NF)-κB signaling components that together coordinate 2-AA-mediated control of transcriptional activity. We found increased NF-κBp65 acetylation levels in 2-AA stimulated cells that are preceded by association of CBP/p300 and increased histone acetyltransferase activity. In contrast, in 2-AA-tolerized cells the protein-protein interaction between p65 and CBP/p300 is disrupted and conversely, the interaction between p50 and co-repressor HDAC1 is enhanced, leading to repression of the pro-inflammatory response. These results highlight how a bacterial QS signaling molecule can establish a link between intracellular signaling and epigenetic reprogramming of pro-inflammatory mediators that may contribute to host tolerance training. These new insights might contribute to the development of novel therapeutic interventions against bacterial infections.