The Composition and Phosphorus Cycling Potential of Bacterial Communities Associated With Hyphae of Penicillium in Soil Are Strongly Affected by Soil Origin

• Published in: Frontiers in microbiology Vol. 10; p. 2951
• Main Authors: Hao, Xiuli, Zhu, Yong-Guan, Nybroe, Ole, Nicolaisen, Mette H
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 08-01-2020
• Subjects: hyphae-associated bacterial community; Microbiology; Penicillium; phosphorus cycling gene; soil microcosm; soil origin; soil origin; hyphae-associated bacterial community; soil microcosm; phosphorus cycling gene; Penicillium
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2019.02951

Intimate fungal-bacterial interactions are widespread in nature. However the main drivers for the selection of hyphae-associated bacterial communities and their functional traits in soil systems remain elusive. In the present study, baiting microcosms were used to recover hyphae-associated bacteria from two
species with different phosphorus-solubilizing capacities in five types of soils. Based on amplicon sequencing of 16S rRNA genes, the composition of bacterial communities associated with
hyphae differed significantly from the soil communities, showing a lower diversity and less variation in taxonomic structure. Furthermore, soil origin had a significant effect on hyphae-associated community composition, whereas the two fungal species used in this study had no significant overall impact on bacterial community structure, despite their different capacities to solubilize phosphorus. However, discriminative taxa and specific OTUs were enriched in hyphae-associated communities of individual
species indicating that each hyphosphere represented a unique niche for bacterial colonization. Additionally, an increased potential of phosphorus cycling was found in hyphae-associated communities, especially for the gene
involved in phosphonate degradation. Altogether, it was established that the two
hyphae represent unique niches in which microbiome assemblage and phosphorus cycling potential are mainly driven by soil origin, with less impact made by fungal identity with a divergent capacity to utilize phosphorus.