Perinatal short‐chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high‐fat diet‐fed adult pigs

• Published in: The FASEB journal Vol. 33; no. 1; pp. 301 - 313
• Main Authors: Le Bourgot, Cindy, Ferret‐Bernard, Stéphanie, Apper, Emmanuelle, Taminiau, Bernard, Cahu, Armelle, Le Normand, Laurence, Respondek, Frédérique, Le Huërou‐Luron, Isabelle, Blat, Sophie
• Format: Journal Article Web Resource
• Language: English
• Published: United States Federation of American Society of Experimental Biology 01-01-2019; Federation of American Societies for Experimental Biology
• Subjects: GLP-1; Life Sciences; Microbiologie; Microbiology; nutritional programming; prebiotic; Prevotella; Sciences du vivant; nutritional programming; GLP-1; prebiotic; Prevotella
• ISSN: 0892-6638; 1530-6860; 1530-6860
• DOI: 10.1096/fj.201800108R

ABSTRACT
Perinatal nutrition programs physiologic and metabolic functions, with consequences on the susceptibility to develop metabolic diseases in adulthood. The microbiota represents a key factor of such programming. We investigated whether perinatal prebiotic [short‐chain fructooligosaccharides (scFOS)] supplementation improved adult metabolic health in association with microbiota changes in pigs used as human model. Sows were supplemented with scFOS or not during the end of gestation and the entire lactation, and offspring received scFOS accordingly during 1 mo after weaning. Pigs were then fed a standard diet for 5 mo, followed by a high‐fat diet for 3 mo once adults. Perinatal scFOS supplementation induced a persistent modulation of the composition of the fecal microbiota in adulthood, notably by increasing the Prevotella genus. Meanwhile, scFOS animals displayed improved capacity to secrete glucagon‐like peptide‐1 and improved pancreas sensitivity to glucose without any changes in peripheral insulin sensitivity. Perinatal scFOS supplementation also increased ileal secretory IgA secretion and alkaline phosphatase activity and decreased TNF‐α expression in adipose tissue. In conclusion, perinatal scFOS supplementation induced long‐lasting modulation of intestinal microbiota and had beneficial consequences on the host physiology in adulthood. Our results highlight the key role of perinatal nutrition on later microbiota and host metabolic adaptation to an unbalanced diet.—Le Bourgot, C., Ferret‐Bernard, S., Apper, E., Taminiau, B., Cahu, A., Le Normand, L., Respondek, F., Le Huerou‐Luron, I., Blat, S. Perinatal short‐chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high‐fat diet‐fed adult pigs. FASEB J. 33, 301–313 (2019). www.fasebj.org