G Protein-Coupled Receptor 101 mRNA Expression in the Mouse Brain: Altered Expression in the Posterior Hypothalamus and Amygdala By Energetic Challenges

• Published in: Journal of neuroendocrinology Vol. 19; no. 1; pp. 34 - 45
• Main Authors: Nilaweera, K. N., Ozanne, D., Wilson, D., Mercer, J. G., Morgan, P. J., Barrett, P.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-01-2007; Blackwell Science; Wiley Subscription Services, Inc
• Subjects: Amygdala - metabolism; Animals; Biological and medical sciences; cocaine and amphetamine-regulated transcript; deprivation of food; Diet; fat containing diet; Food Deprivation; Fundamental and applied biological sciences. Psychology; Hypothalamus, Posterior - metabolism; Injections; leptin; Leptin - administration & dosage; Leptin - genetics; Male; Medical sciences; Mice; Mice, Inbred C57BL; Mice, Inbred Strains; Mice, Obese; Mutation; Nerve Tissue Proteins - metabolism; Neuropharmacology; Pharmacology. Drug treatments; pro-opiomelanocortin; Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease); Psychology. Psychoanalysis. Psychiatry; Psychopharmacology; Receptors, G-Protein-Coupled - metabolism; Vertebrates: endocrinology; Amphetamine derivatives; Amygdala; Psychotropic; Central nervous system; Prohormone; Encephalon; Ester; Proopiocortin; Amfetamine; Drug of abuse; G protein; Biological receptor; Posterior hypothalamus; CNS stimulant; fat containing diet; pro-opiomelanocortin; Rodentia; Basal ganglion; Adipokine; cocaine and amphetamine-regulated transcript; deprivation of food; Gene expression; Feeding; Vertebrata; Mammalia; Diet; Mouse; Animal; Leptin; Cocaine; Restricted diet
• ISSN: 0953-8194; 1365-2826
• DOI: 10.1111/j.1365-2826.2006.01502.x

GPCR101 is a recently identified orphan G protein‐coupled receptor (GPCR) expressed abundantly in the human and mouse hypothalamus. In the absence of a ligand, a direct approach to determine the function(s) of this receptor is not possible. However, clues to the possible functions of GPCR101 may yield from information on the distribution of the receptor and the effect of in vivo manipulation upon the expression level of the receptor. In situ hybridisation on mouse brain sections revealed GPCR101 expression in a number of nuclei, including the amygdala, lateral parabrachial nucleus and nucleus of the solitary tract, as well as in the arcuate nucleus, posterior hypothalamus and paraventricular nucleus of the hypothalamus. Food‐deprivation was found to increase GPCR101 mRNA level in the posterior hypothalamus and amygdala. In obese mice bearing the ob gene mutation, GPCR101 mRNA level decreased in the posterior hypothalamus and remained unaltered in the amygdala. By contrast, in both nuclei, GPCR101 mRNA level did not change significantly in obese ob/ob mice after intraperitoneal injection of leptin or in mice fed with a high fat diet. These data suggest that GPCR101 mRNA expression in the posterior hypothalamus and amygdala is regulated by a factor(s) other than leptin. Dual in situ hybridisation was used to establish the relationship between GPCR101 and neuropeptides expressed in the hypothalamus. In the arcuate nucleus, GPCR101 mRNA was expressed in approximately half of the population of neurones expressing the mRNA for the anorexigenic neuropeptide, pro‐opiomelanocortin, which suggests a potential functional relationship.