Immunophenotyping lymphocyte and acute phase proteins in canine X-linked muscular dystrophy

• Published in: Anais da Academia Brasileira de Ciências Vol. 90; no. 3; pp. 2977 - 2990
• Main Authors: Abreu, Dilayla K DE, Monteiro, Janaina M, Souza, Carolina C, Karam, Rafael G, Fernandes, Renata A, Lessa, Thais B, Fagliari, José J, Miglino, Maria Angelica, Ambrosio, Carlos Eduardo
• Format: Journal Article
• Language: English
• Published: Brazil Academia Brasileira de Ciências 01-09-2018
• Subjects: Acute phase protein; Acute-Phase Proteins - immunology; Age Factors; Animals; Blood Proteins - immunology; Disease Models, Animal; Dog Diseases - immunology; Dogs; Dystrophic model; Flow Cytometry; Immune behavior; Immunophenotyping; Inflammation - immunology; Inflammatory response; MULTIDISCIPLINARY SCIENCES; Muscular dystrophy; Muscular Dystrophy, Duchenne - immunology; Serum protein; Inflammatory response; Acute phase protein; Immune behavior; Serum protein; Dystrophic model; Muscular dystrophy
• ISSN: 0001-3765; 1678-2690; 1678-2690
• DOI: 10.1590/0001-3765201820170838

Duchenne Muscular Dystrophy (DMD) is the most common X-linked muscular disease affecting humans. The Golden Retriever Muscular Dystrophy model (GRMD) is considerthe most suitable for several studies. This assay aims to quantify lymphocyte subpopulations CD4, CD5, and CD8, and standardize, the serum electrophoretic profile, to understand their contribution to the pathologic process in normal Golden Retriever dogs (GR group) and dystrophic´s (GRMD group), through the umbilical cord blood, in dogs aged from 2 to 3 months (GR II and GRMD II), and in dogs over 1 year of age (GR III and GRMD III). No significant differences were observed between the CD8+ lymphocyte subpopulations of the groups studied. The CD4+ and CD5+ lymphocyte subpopulations were significantly higher in the GRMD III group compared to the GR III group. Twenty-two different proteins in the gel were identified. The serum concentrations of the proteins belonging to the GR I and GRMD I groups were significantly lower than those of the other groups. We show that expression of acute phase proteins are worst during the aging of the dogs. We hope to expand knowledge to better understand the GRMD model and the translational data.