Arginine vasopressin does not alter mucosal tissue oxygen tension and oxygen supply in an acute endotoxemic pig model

• Published in: Intensive care medicine Vol. 32; no. 1; pp. 170 - 174
• Main Authors: KNOTZER, Hans, MAIER, Stephan, PAJK, Werner, DÜNSER, Martin W, HASIBEDER, Walter R, HAUSDORFER, Hans, BRANDNER, Julia, TORGERSEN, Christian, ULMER, Hanno, FRIESENECKER, Barbara, IANNETTI, Claudia
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 2006; Berlin Springer Nature B.V
• Subjects: Analysis of Variance; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Anesthesiology; Animals; Arginine Vasopressin - administration & dosage; Arginine Vasopressin - pharmacology; Biological and medical sciences; Catheters; Causes of; Critical care; Diagnosis; Diseases of the respiratory system; Dosage and administration; Dose-Response Relationship, Drug; Drug dosages; Drug therapy; Emergency and intensive respiratory care; Hemoglobin; Hypoxia; Intensive care medicine; Intestinal Mucosa - blood supply; Intestinal Mucosa - drug effects; Intestinal Mucosa - metabolism; Jejunum; Jejunum - blood supply; Medical examination; Medical sciences; Microcirculation; Oxygen - metabolism; Oxygen saturation; Partial Pressure; Pulmonary arteries; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Random Allocation; Shock, Septic - drug therapy; Spectrophotometry; Splanchnic Circulation; Swine; Vasoconstrictor Agents - administration & dosage; Vasoconstrictor Agents - pharmacology; Vasopressin; Austria; Germany; Oxygen; Intensive care; Digestive system; Tissue oxygen tension; Arginine-vasopressin; Reflectance spectrophotometry; Supply; Small intestine; Neuropeptide; Vasopressin; Pig; Vertebrata; Mammalia; Jejunum; Arginine; Aminoacid; Neurohypophyseal hormone; Artiodactyla; Spectrophotometry; Ungulata; Endotoxemia; Resuscitation
• ISSN: 0342-4642; 1432-1238
• DOI: 10.1007/s00134-005-2858-z

To determine the effects of increasing dosages of continuously infused arginine-vasopressin (AVP) on mucosal tissue oxygen tension and oxygen supply in an auto-perfused, innervated jejunal segment in an acute endotoxic porcine model.
Prospective, randomized, experimental study.
University hospital animal research laboratory.
Jejunal mucosal tissue PO2 was measured employing two Clark-type surface oxygen electrodes. Oxygen saturation of jejunal microvascular hemoglobin was determined by tissue reflectance spectrophotometry. Systemic hemodynamic variables, mesenteric-venous and systemic acid base and blood gas variables and lactate measurements were recorded. Measurements were performed at baseline, after E. coli lipopolysaccharide (LPS) administration and at 20 min intervals during incremental AVP infusion (n=8; 0.014, 0.029, 0.057, 0.114 and 0.229 IU kg(-1) h(-1), respectively) or infusion of saline (n =8).
LPS infusion leads to a significant (P<0.05) decrease of mucosal tissue oxygen tension (PO2muc, 24+/-3 to 12+/-2 mmHg) and microvascular hemoglobin oxygen saturation (HbO2, 38+/-4 to 21+/-4%). Mesenteric venous lactate level increased (2.4+/-0.3 to 4.7+/-1.7 mmol l(-1)), while mesenteric venous pH decreased (7.38+/-0.02 to 7.26+/-0.12), indicating tissue hypoxia. AVP significantly increased mean arterial pressure (MAP, 81+/-15 to 97+/-17 at 0.057 IU kg(-1) h(-1)). No differences in jejunal mucosal oxygenation occurred between study groups at any dosage during the experimental protocol.
AVP administration did not further compromise mucosal tissue oxygen tension and oxygen supply in the acute phase of endotoxic pigs.