Progression of lesions in the respiratory tract of broilers after single infection with Escherichia coli compared to superinfection with E. coli after infection with infectious bronchitis virus

• Published in: Veterinary immunology and immunopathology Vol. 127; no. 1; pp. 65 - 76
• Main Authors: Dwars, R. Marius, Matthijs, Mieke G.R., Daemen, Angeline J.J.M., van Eck, Jo H.H., Vervelde, Lonneke, Landman, Wil J.M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-01-2009; Amsterdam: Elsevier
• Subjects: Air Sacs - microbiology; Air Sacs - pathology; Air Sacs - virology; Airsacculitis; Animals; antigen detection; Antigens, Bacterial - metabolism; Antigens, Viral - metabolism; broiler chickens; Bronchi - microbiology; Bronchi - pathology; Bronchi - virology; Chickens; Coronavirus Infections - complications; Coronavirus Infections - pathology; Coronavirus Infections - veterinary; disease resistance; edema; Escherichia coli; Escherichia coli - immunology; Escherichia coli - isolation & purification; Escherichia coli Infections - complications; Escherichia coli Infections - pathology; Escherichia coli Infections - veterinary; Escherichia infections; experimental infection; histopathology; immunohistochemistry; infectious bronchitis; Infectious bronchitis virus; Infectious bronchitis virus - immunology; Infectious bronchitis virus - isolation & purification; Infectious bronchitis virus - pathogenicity; inflammation; lesions (animal); Lung - microbiology; Lung - pathology; Lung - virology; Pneumonia; Poultry Diseases - microbiology; Poultry Diseases - pathology; Poultry Diseases - virology; respiratory tract diseases; Respiratory Tract Infections - microbiology; Respiratory Tract Infections - pathology; Respiratory Tract Infections - veterinary; Respiratory Tract Infections - virology; Superinfection; Superinfection - microbiology; Superinfection - pathology; Superinfection - veterinary; Superinfection - virology; Trachea - microbiology; Trachea - pathology; Trachea - virology; Tracheitis; Airsacculitis; Pneumonia; Superinfection; Tracheitis; Escherichia coli; Infectious bronchitis virus
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/j.vetimm.2008.09.019

The progression of
Escherichia coli lesions was studied in the respiratory tract of 4-week-old commercial broilers. Lesions were induced after a single intratracheal
E. coli infection, and after an infection with
E. coli preceded 5 days earlier by an oculo-nasal and intratracheal infectious bronchitis virus (IBV) infection of either the virulent M41 strain or the H120 vaccine strain. Trachea, lung and thoracic airsac lesions were examined macroscopically and microscopically. Tissue samples were taken at 3
h post-inoculation (hpi), and 1, 2, 4 and 7 days post-inoculation (dpi) with
E. coli. The location of both pathogens was assessed by immunohistochemistry. Single
E. coli inoculation induced pneumonia and airsacculitis; in case it was preceded by IBV infection, the same macroscopical lesions and also viral tracheitis were found. No clear difference existed between the single and dual infected birds with respect to inflammatory reactions in the lung, which had disappeared within 7 days, except for the presence of more follicles in dual infected birds. IBV antigen was detected in secondary bronchi and airsacs up to 2
dpi and in the trachea up to 4
dpi.
E. coli bacteria were found in the tracheal lumen included in purulent material, the parabronchi and airsacs. In lung tissue
E. coli antigen was found up to 4
dpi. No clear difference existed between single and dual inoculated birds regarding the presence of
E. coli in the lung. In the airsacs, a few bacteria were found from 0.5
hpi up to 4
dpi in
E. coli and IBV–
E. coli inoculated birds. Although both pathogens were cleared beyond detection at 7
dpi, in IBV–
E. coli inoculated birds lesions in the airsac persisted, in contrast to broilers inoculated with
E. coli only. In the present study it is shown that 4-week-old broilers are not resistant to intratracheal
E. coli inoculation, however, these birds can overcome the induced
E. coli infection within a short time span. Moreover, a preceding infection with vaccine or virulent IBV does not seem to impair the clearance of
E. coli in the respiratory tract of broilers, but rather induces an exaggerated inflammatory response in the airsacs only, which seems to be the mechanism behind the pattern of airsacculitis in commercial poultry in the field.