Structure analysis of a bioactive heteropolysaccharide from Schisandra chinensis (Turcz.) Baill

• Published in: Carbohydrate polymers Vol. 103; pp. 488 - 495
• Main Authors: Zhao, Ting, Mao, Guanghua, Zhang, Min, Feng, Weiwei, Mao, Riwen, Zhu, Yang, Gu, Xiaoyun, Li, Qian, Yang, Liuqing, Wu, Xiangyang
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 15-03-2014; Elsevier
• Subjects: Antineoplastic agents; Applied sciences; Biological and medical sciences; Carbohydrate Conformation; Chain conformation; Chemotherapy; Exact sciences and technology; Medical sciences; Natural polymers; Pharmacology. Drug treatments; Physicochemistry of polymers; Polysaccharide; Polysaccharides - analysis; Schisandra - chemistry; Schisandra chinensis (Turcz.) Baill; Starch and polysaccharides; Structural elucidation; Structure–activity correlation; Structural elucidation; Chain conformation; Structure–activity correlation; Polysaccharide; Schisandra chinensis (Turcz.) Baill; Antineoplastic agent; Circular dichroism spectrum; Molecular structure; Temperature effect; Radius of gyration; Structure―activity correlation; Medicinal plant; Chinese medicine; Folk medicine; Structure activity relation; Dicotyledones; Magnoliaceae; Angiospermae; pH; Experimental study; Biological activity; Immunomodulator; Plant origin; Oside polymer; Spermatophyta; Aqueous solution; Conformation
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.12.058

Our previous study revealed that Schisandra polysaccharide (SCPP11) exerted excellent antitumor and immunomodulatory activities. In this investigation, the structure of SCPP11 was elucidated. The results indicated that SCPP11 has a backbone that is composed of 1,4-disubsituted-β-gal, 1,4-disubsituted-α-glu, 1,6-disubsituted-β-man and 1,4,6-disubsituted-α-gal. The branches was composed of 1,4-disubsituted-α-glu and 1-substitued-β-glu. The Mw and (rg2)z1/2 of the polymer molecules in 0.1M NaCl were 1.793×104Da and 11nm, respectively. The exponent of (rg2)z1/2 versus Mw (0.39) suggested SCPP11 adopted a globular conformation with seldom random coil. Circular dichroism analysis revealed the presence of ordered structures in SCPP11. AFM and TEM further confirmed the agglomerated morphologies of SCPP11. In addition, it inferred the agglomerated conformation, branching structure and flexibility of chain are beneficial for exerting excellent activities. This information will be helpful in the recognition of biological systems for polysaccharides and the selection of active polysaccharide.