Vaccination of bovines with recombinant Boophilus Yolk pro-Cathepsin

• Published in: Veterinary Immunology and Immunopathology Vol. 114; no. 3; pp. 341 - 345
• Main Authors: Leal, Alexandre T., Seixas, Adriana, Pohl, Paula C., Ferreira, Carlos A.S., Logullo, Carlos, Oliveira, Pedro L., Farias, Sandra E., Termignoni, Carlos, da Silva Vaz, Itabajara, Masuda, Aoi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-12-2006
• Subjects: Animals; Antibody Formation; Aspartic Acid Endopeptidases - genetics; Aspartic Acid Endopeptidases - immunology; Aspartic proteinase; aspartic proteinases; Blotting, Western - veterinary; Boophilus; Boophilus microplus; Bovine; BYC; cathepsins; Cattle; cattle diseases; Cattle Diseases - immunology; Cattle Diseases - parasitology; Cattle Diseases - prevention & control; Cytokine; egg yolk; Enzyme Precursors - genetics; Enzyme Precursors - immunology; Enzyme-Linked Immunosorbent Assay - veterinary; Escherichia coli; Female; Immunoglobulin G - blood; Ixodidae - immunology; ova; recombinant antigens; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Tick Infestations - immunology; Tick Infestations - parasitology; Tick Infestations - prevention & control; Tick Infestations - veterinary; Tick vaccine; ticks; vaccination; Vaccination - veterinary; Boophilus microplus; Aspartic proteinase; Bovine; BYC; Tick vaccine; Cytokine
• ISSN: 0165-2427; 1873-2534; 1365-2567
• DOI: 10.1016/j.vetimm.2006.08.011

Boophilus Yolk pro-Cathepsin (BYC) is an aspartic proteinase found in
Boophilus microplus eggs that is involved in the embryogenesis and has been tested as antigen to compose an anti-tick vaccine. The vaccine potential of a recombinant BYC expressed in
Escherichia coli (rBYC) was investigated. rBYC was purified and used to immunize Hereford cattle. The sera of bovines immunized with rBYC recognized the native BYC with a titer ranging from 125 to 4000. Furthermore, immunized bovines challenged with 20,000 larvae presented an overall protection of 25.24%. The partial protection obtained against
B. microplus infestation with the recombinant protein immunization was similar to the already described for native BYC immunization.