Effect of storage on bioactivity of an Algerian spice “paprika”: optimization of phenolic extraction and study of antioxidant and antibacterial activities

• Published in: Food science and biotechnology Vol. 33; no. 4; pp. 999 - 1011
• Main Authors: Bouziane-Ait Bessai, Keltoum, Brahmi-Chendouh, Nabila, Brahmi, Fatiha, Dairi, Sofiane, Mouhoubi, Khokha, Kermiche, Ferriel, Bedjaoui, Kenza, Madani, Khodir, Boulekbache-Makhlouf, Lila
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01-03-2024; Springer Nature B.V; 한국식품과학회
• Subjects: Ambient temperature; Antibacterial activity; Antioxidants; Biological activity; Chemistry; Chemistry and Materials Science; Ethanol; Flavonoids; Food Science; Freezing; Gallic acid; Irradiation; Nutrition; Paprikas; Phenolic compounds; Phenols; Refrigeration; Research Article; Response surface methodology; 식품과학; Storage; Box–Behnken design; Biological activities; Paprika; Phenolic compounds; Ultrasound-assisted extraction
• ISSN: 1226-7708; 2092-6456; 2092-6456
• DOI: 10.1007/s10068-023-01375-1

The effect of different storage methods (ambient temperature (A), refrigeration at 4 °C (R) and freezing at − 18 °C (F)), on the phytochemistry of an Algerian spice (paprika powder), was assessed. The optimized extract was obtained under the optimum conditions of ultrasound-assisted extraction (UAE) using response surface methodology (RSM) coupled with a Box–Behnken Design (BBD). This extract was evaluated for its total phenolics content (TPC), total flavonoids content (TFC) and its antioxidant and antibacterial activities. Under the optimum conditions (5 min for the irradiation time, 40% for the amplitude, 80% for ethanol concentration and 50% for solid–liquid ratio) the TPC was 12.23 ± 1.01 mg Gallic Acid Equivalent/gram of Dried Powder (mg GAE/g DP) which is very close with experimental assay. The TPC are better preserved at A whereas TFC and the antioxidant activity at F, and the antibacterial activity depend on the storage methods and the strains tested.