High Performance Liquid Chromatographic Determination of Residual Monomer Released from Heat-Cured Acrylic Resin. An In Vivo Study

• Published in: Journal of prosthodontics Vol. 22; no. 5; pp. 358 - 361
• Main Authors: Singh, Raghuwar D., Gautam, Rupali, Siddhartha, Ramashanker, Singh, Balendra P., Chand, Pooran, Sharma, Vinod P., Jurel, Sunit K.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01-07-2013; Wiley Subscription Services, Inc
• Subjects: Acrylic Resins - analysis; Acrylic Resins - chemistry; Aged; Chromatography, High Pressure Liquid - methods; Complete denture; Dental Materials - analysis; Dental Materials - chemistry; Dentistry; Denture Bases; Denture Design; Denture, Complete; Diffusion; Follow-Up Studies; heat-cured acrylic resin; high performance liquid chromatography; Hot Temperature; Humans; Methylmethacrylate - analysis; Methylmethacrylate - chemistry; Middle Aged; Mouth, Edentulous - rehabilitation; Polymerization; Pressure; residual monomer methyl methacrlylate; saliva; Saliva - chemistry; saliva; heat-cured acrylic resin; high performance liquid chromatography; residual monomer methyl methacrlylate; Complete denture
• ISSN: 1059-941X; 1532-849X
• DOI: 10.1111/jopr.12004

Purpose
Heat‐polymerized acrylic resins are used in dentistry for complete denture fabrication. Despite the polymerization method, conversion of monomer into polymer is often incomplete with free or unreacted residual monomer remaining in the polymerized resin. The aim of this study was to determine the amount of residual monomeric methyl methacrylate (MMA) leaching in the saliva of patients wearing complete dentures in their postinsertion period.
Materials and Methods
Thirty edentulous participants as first‐time complete denture wearers (age 60 to 65 years) were selected. All the prostheses were fabricated using a similar standard technique with a heat‐cured acrylic resin denture base material. Saliva samples were collected at time intervals of 1 hour, 1 day, and 3 days postdenture insertion. Participants were asked to discharge saliva every 30 seconds into a pre‐weighed screw‐capped container for a 5‐minute period. MMA levels were measured using high performance liquid chromatography. Data were analyzed by ANOVA and Tukey‐HSD.
Results
The maximum concentration of monomer released into saliva peaked 1 day after insertion of the complete dentures. The mean (SD) MMA content was 0.04 ± 0.01 (μg/ml) 1 hour after insertion, and 0.3 ± 0.09 (μg/ml), and 0.05 ± 0.01 (μg/ml) on the first and third days postinsertion, respectively.
Conclusions
Although the released monomeric MMA was not at toxic levels, it could potentially sensitize complete denture patients or elicit an allergic reaction. The risk of the residual material as a primary irritant for a sensitizing reaction could be minimized by immersion of the denture in water for 24 hours before insertion.