Influence of Trans Fat on Skin Damage in First-Generation Rats Exposed to UV Radiation

• Published in: Photochemistry and photobiology Vol. 91; no. 2; pp. 424 - 430
• Main Authors: Barcelos, Raquel Cristine S., Vey, Luciana T., Segat, Hecson Jesser, Benvegnú, Dalila M., Trevizol, Fabíola, Roversi, Karine, Roversi, Katiane, Dias, Verônica T., Dolci, Geisa S., Kuhn, Fábio T., Piccolo, Jaqueline, CristinaVeit, Juliana, Emanuelli, Tatiana, Bürger, Marilise E.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01-03-2015
• Subjects: Animals; Antioxidants; Antioxidants - metabolism; Catalase - metabolism; Dietary Supplements; Enzymes; Female; Hydrogenation; Lipids; Mitochondria; Mitochondria - drug effects; Mitochondria - radiation effects; Plant Oils - administration & dosage; Pregnancy; Protein Carbonylation - drug effects; Protein Carbonylation - radiation effects; Rats; Rats, Wistar; Reactive Oxygen Species - metabolism; Skin - chemistry; Skin - drug effects; Skin - metabolism; Skin - radiation effects; Skin Aging - drug effects; Skin Aging - radiation effects; Soybean Oil - administration & dosage; Superoxide Dismutase - metabolism; Trans fats; Ultraviolet radiation; Ultraviolet Rays; Vegetable oils
• ISSN: 0031-8655; 1751-1097
• DOI: 10.1111/php.12414

The influence of trans fatty acids (TFA) on lipid profile, oxidative damage and mitochondrial function in the skin of rats exposed to ultraviolet radiation (UVR) was assessed. The first‐generation offspring of female Wistar rats supplemented from pregnancy with either soybean oil (C‐SO, rich in n–6 FA; control group) or hydrogenated vegetable fat (HVF, rich in TFA) were continued with the same supplements until adulthood, when half of each group was exposed to UVR for 12 weeks. The HVF group showed higher TFA cutaneous incorporation, increased protein carbonyl (PC) levels, decreased functionality of mitochondrial enzymes and antioxidant defenses of the skin. After UVR, the HVF group showed increased skin thickness and reactive species (RS) generation, with decreased skin antioxidant defenses. RS generation was positively correlated with skin thickness, wrinkles and PC levels. Once incorporated to skin, TFA make it more susceptible to developing UVR‐induced disorders.
Metabolism of trans fat by COX and LOX: The dietary intake of trans fat favors the synthesis of biologically active prostanoids produced during inflammatory processes. After ultraviolet radiation exposure, trans fat supplementation, which is rich in trans fatty acids (TFA), increases the skin thickness, reactive species generation and decreases the skin antioxidant defenses.