Two different spectrofluorimetric methods for simultaneous determination of gemfibrozil and rosiglitazone in human plasma

• Published in: Talanta (Oxford) Vol. 82; no. 5; pp. 1708 - 1716
• Main Authors: El-Din, Mohie M.K. Sharaf, Attia, Khalid A.M., Nassar, Mohamed W.I., Kaddah, Mohamed M.Y.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15-10-2010; Elsevier
• Subjects: Analytical chemistry; Buffers; Calibration; Chemistry; Exact sciences and technology; Gemfibrozil; Gemfibrozil - blood; Human plasma; Humans; Hydrogen-Ion Concentration; Hypoglycemic Agents - blood; In Vitro Techniques; Reference Standards; Reproducibility of Results; Rosiglitazone; Sensitivity and Specificity; Solutions; Spectrometric and optical methods; Spectrometry, Fluorescence - methods; Synchronous fluorescence spectrometry; Thiazolidinediones - blood; Vierodt's method; Rosiglitazone; Human plasma; Vierodt's method; Synchronous fluorescence spectrometry; Gemfibrozil; Human; Plasma; Fluorescence; Concentration; Method; Wavelength; Fluorescence spectrometry; Binary mixture; Synchronous; Signal; Detection limit; Simultaneous measurement; Yield; Technique
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2010.07.058

Two accurate, reliable, and highly sensitive spectrofluorimetric methods were developed for simultaneous determination of binary mixture gemfibrozil and rosiglitazone in human plasma without prior separation steps. The first method is based on synchronous fluorescence spectrometry using double scans. At Δ
λ
=
27
nm, gemfibrozil yields detectable signal that is independent of the presence of rosiglitazone. Similarly, at Δ
λ
=
120
nm the signal of rosiglitazone is not influenced by the presence of gemfibrozil. Signals at two wavelengths, 301 (Δ
λ
=
27
nm) and 368
nm (Δ
λ
=
120
nm) vary linearly with gemfibrozil and rosiglitazone concentrations over the range 100–700
ng
mL
−1 (for gemfibrozil) and 20–140
ng
mL
−1 (for rosiglitazone), respectively. The limits of detection (LOD) were 2.3 and 2.72
ng
mL
−1 for gemfibrozil and rosiglitazone, respectively. The second method is based on the technique of simultaneous equations (Vierodt's method), in which 258
nm was selected as the excitation wavelength. Two equations are constructed based on the fact that at (
λ
Em
2
=
302

nm
of gemfibrozil) and (
λ
Em
2
=
369

nm
of rosiglitazone) the fluorescence of the mixture is the sum of the individual fluorescence of gemfibrozil and rosiglitazone. The limits of detection (LOD) were 28.1 and 23.63
ng
mL
−1 for gemfibrozil and rosiglitazone, respectively. The proposed methods were successfully applied for the determination of the two compounds in synthetic mixtures and in human plasma with a good recovery.