Bioinspired negatively charged calcium phosphate nanocarriers for cardiac delivery of MicroRNAs

• Published in: Nanomedicine (London, England) Vol. 11; no. 8; pp. 891 - 906
• Main Authors: Di Mauro, Vittoria, Iafisco, Michele, Salvarani, Nicolò, Vacchiano, Marco, Carullo, Pierluigi, Ramírez-Rodríguez, Gloria Belén, Patrício, Tatiana, Tampieri, Anna, Miragoli, Michele, Catalucci, Daniele
• Format: Journal Article
• Language: English
• Published: England Future Medicine Ltd 01-04-2016
• Subjects: Animals; Biocompatibility; Biocompatible Materials - chemistry; Biocompatible Materials - metabolism; calcium phosphate nanoparticles; Calcium phosphates; Calcium Phosphates - chemistry; Calcium Phosphates - metabolism; cardiomyocytes; Cell Line; Cells, Cultured; Deoxyribonucleic acid; DNA; Drug Carriers - chemistry; Drug Carriers - metabolism; Drug Delivery Systems; Gene Transfer Techniques; Humans; Lipids; Mice, Inbred C57BL; microRNA; MicroRNAs; MicroRNAs - administration & dosage; Myocytes, Cardiac - metabolism; nanomedicine; Nanoparticles; Nanoparticles - chemistry; Nanoparticles - metabolism; Sodium; cardiomyocytes; microRNA; nanomedicine; calcium phosphate nanoparticles; drug-delivery systems
• ISSN: 1743-5889; 1748-6963
• DOI: 10.2217/nnm.16.26

To develop biocompatible and bioresorbable negatively charged calcium phosphate nanoparticles (CaP-NPs) as an innovative therapeutic system for the delivery of bioactive molecules to the heart.
CaP-NPs were synthesized via a straightforward one-pot biomineralization-inspired protocol employing citrate as a stabilizing agent and regulator of crystal growth. CaP-NPs were administered to cardiac cells
and effects of treatments were assessed. CaP-NPs were administered
and delivery of microRNAs was evaluated.
CaP-NPs efficiently internalized into cardiomyocytes without promoting toxicity or interfering with any functional properties. CaP-NPs successfully encapsulated synthetic microRNAs, which were efficiently delivered into cardiac cells
and
.
CaP-NPs are a safe and efficient drug-delivery system for potential therapeutic treatments of polarized cells such as cardiomyocytes.