Role of Iron Uptake Systems in Pseudomonas aeruginosa Virulence and Airway Infection

• Published in: Infection and immunity Vol. 84; no. 8; pp. 2324 - 2335
• Main Authors: Minandri, Fabrizia, Imperi, Francesco, Frangipani, Emanuela, Bonchi, Carlo, Visaggio, Daniela, Facchini, Marcella, Pasquali, Paolo, Bragonzi, Alessandra, Visca, Paolo
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01-08-2016
• Subjects: Animals; Bacterial Infections; Disease Models, Animal; Humans; Iron - metabolism; Mice; Mutation; Oligopeptides - metabolism; Phenotype; Pseudomonas aeruginosa; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - physiology; Pseudomonas Infections - metabolism; Pseudomonas Infections - microbiology; Reproductive Tract Infections - metabolism; Reproductive Tract Infections - microbiology; Signal Transduction; Transferrin - metabolism; Virulence
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.00098-16

Pseudomonas aeruginosa is a leading cause of hospital-acquired pneumonia and chronic lung infections in cystic fibrosis patients. Iron is essential for bacterial growth, and P. aeruginosa expresses multiple iron uptake systems, whose role in lung infection deserves further investigation. P. aeruginosa Fe(3+) uptake systems include the pyoverdine and pyochelin siderophores and two systems for heme uptake, all of which are dependent on the TonB energy transducer. P. aeruginosa also has the FeoB transporter for Fe(2+) acquisition. To assess the roles of individual iron uptake systems in P. aeruginosa lung infection, single and double deletion mutants were generated in P. aeruginosa PAO1 and characterized in vitro, using iron-poor media and human serum, and in vivo, using a mouse model of lung infection. The iron uptake-null mutant (tonB1 feoB) and the Fe(3+) transport mutant (tonB1) did not grow aerobically under low-iron conditions and were avirulent in the mouse model. Conversely, the wild type and the feoB, hasR phuR (heme uptake), and pchD (pyochelin) mutants grew in vitro and caused 60 to 90% mortality in mice. The pyoverdine mutant (pvdA) and the siderophore-null mutant (pvdA pchD) grew aerobically in iron-poor media but not in human serum, and they caused low mortality in mice (10 to 20%). To differentiate the roles of pyoverdine in iron uptake and virulence regulation, a pvdA fpvR double mutant defective in pyoverdine production but expressing wild-type levels of pyoverdine-regulated virulence factors was generated. Deletion of fpvR in the pvdA background partially restored the lethal phenotype, indicating that pyoverdine contributes to the pathogenesis of P. aeruginosa lung infection by combining iron transport and virulence-inducing capabilities.