Lactic acid bacteria isolated from fermented flour of finger millet, its probiotic attributes and bioactive properties

• Published in: Annals of microbiology Vol. 69; no. 2; pp. 79 - 92
• Main Authors: Divisekera, Divisekera Mudiyanselage Wasundara Devanmini, Samarasekera, Jayanetti Koralalage Ramani Radhika, Hettiarachchi, Chamari, Gooneratne, Jaanaki, Choudhary, Muhammad Iqbal, Gopalakrishnan, Subramaniam, Wahab, Atia-tul
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 24-11-2018; Springer; Springer Nature B.V
• Subjects: Acids; Adenocarcinoma; Amino acids; Antibiotics; Applied Microbiology; Bacteria; Bactericidal activity; Bile; Biochemical characteristics; Biomedical and Life Sciences; Cholesterol; Colorectal cancer; Colorectal carcinoma; Drug resistance in microorganisms; Eleusine coracana; Ethylenediaminetetraacetic acid; Fermented food; Flour; Gastric juice; Hydrophobicity; Lactic acid; Lactic acid bacteria; Life Sciences; Medical Microbiology; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Multidrug resistance; Mycology; Original Article; Phenols; Probiotics; RNA; rRNA; Strains (organisms); Tetracycline; Tetracyclines; Tumor cell lines; Yogurt; Sri Lanka; United Kingdom; Probiotics; Lactic acid bacteria; Bioactivity; Fermented finger millet flour
• ISSN: 1590-4261; 1869-2044
• DOI: 10.1007/s13213-018-1399-y

This study aims to isolate and identify lactic acid bacteria from fermented flour of selected finger millet varieties grown in Sri Lanka and to evaluate their probiotic attributes and bioactive properties in vitro. Fifteen lactic acid bacteria were isolated from three varieties of fermented finger millet flour namely
ravi
,
raavana
and
oshadha
. These isolates were screened for phenotypical and biochemical characteristics. The selected isolates were identified by 16 S rRNA sequencing as
Bacillus cereus
(five strains),
Streptococcus lutetiensis
,
Lactobacillus plantarum
,
Lactobacillus fermentum
(two strains),
Brevibacillus borstelensis
,
Paenibacillus
species,
Lactococcus lactis
subspecies
lactis
,
Enterococcus faecium
,
Pediococcus acidilactici
, and
Enterococcus lactis
, and their partial sequences were deposited in GenBank. Among them, five isolates including two isolates,
L. plantarum
MF405176.1 and
L. fermentum
MF033346.1 isolated from
ravi
; two isolates,
L. lactis
MF480428.1 and
E. faecium
MF480431.1 isolated from
raavana
; and
P. acidilactici
MF480434.1 isolated from
oshadha
varieties respectively, exhibited in vitro safety attributes and could tolerate acid, gastric juice, bile, salt, phenol, and temperature under simulated gastric conditions, and also were susceptible to antibiotics tested. Further, they demonstrated bactericidal activity against both drug-sensitive and multidrug-resistant pathogens. Among the selected isolates,
L. plantarum
MF405176.1 demonstrated highest hydrophobicity and adhesion to both colon colorectal adenocarcinoma and colon colorectal carcinoma cell lines.
L. lactis
subspecies
lactis
MF480428.1 exhibited the highest auto-aggregation and 2, 2, diphenyl-1-pricrylhydrazyl free radical scavenging activity.
P. acidilactici
MF480434.1 demonstrated the lowest IC
50
values against HCT-116 and HT-29 cells. None of the LAB isolates could assimilate > 10% cholesterol in vitro
.