Diversity of anti-haemostatic proteins in the salivary glands of Rhodnius species transmitters of Chagas disease in the greater Amazon

• Published in: Journal of proteomics Vol. 74; no. 9; pp. 1664 - 1672
• Main Authors: Bussacos, Ana C.M., Nakayasu, Ernesto S., Hecht, Mariana M., Parente, Juliana A., Soares, Célia M.A., Teixeira, Antônio R.L., Almeida, Igor C.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 24-08-2011
• Subjects: Animals; blood; Chagas Disease - parasitology; Chagas Disease - transmission; Gene Expression Profiling; glutathione; Glutathione Transferase - analysis; Glutathione Transferase - genetics; Haematophagous insects; Humans; Inositol Polyphosphate 5-Phosphatases; Insect Proteins - analysis; Insect Vectors - chemistry; Insect Vectors - pathogenicity; Insecticide Resistance - genetics; insecticides; insects; Lipocalins - analysis; Lipocalins - genetics; myo-inositol; Phosphoric Monoester Hydrolases - analysis; Phosphoric Monoester Hydrolases - genetics; platelet aggregation; Platelet Aggregation Inhibitors - analysis; proteins; Proteomics; Rhodnius; Rhodnius - chemistry; Rhodnius - parasitology; Rhodnius robustus; Salivary glands; Salivary Glands - chemistry; Salivary Glands - parasitology; sialome; transcriptome; Transcriptomics; Trypanosoma cruzi; Wasp Venoms - analysis; Wasp Venoms - genetics; South America, Amazon R; Rb; Rr; Salivary glands; DTT; Transcriptomics; Proteomics; GST; 2-DE; FA; ACN; LC–MS/MS; Haematophagous insects
• ISSN: 1874-3919; 1876-7737
• DOI: 10.1016/j.jprot.2011.06.022

The triatomines in the tribe Rhodniini are the main vectors of the
Trypanosoma cruzi to humans in recent outbreaks of acute Chagas disease in the Amazon. These insects dwelling in palm trees do not colonize the human domicile. Their success to transmit the infection relies partially on the efficacy of their salivary gland apparatuses. Here we show the transcriptome of the
Rhodnius brethesi and
Rhodnius robustus salivary glands, comprising 56 and 122 clusters, respectively. Approximately one third of these clusters are described for the first time. The LC–MS/MS analysis identified 123 and 111 proteins in
R. brethesi and
R. robustus sialome, respectively. Noteworthy, lipocalin platelet aggregation inhibitors, inositol polyphosphate 5-phosphatases, and Kazal domain proteins, which are essential for the insect's successful acquisition of blood meals, were found in our analysis. Moreover, glutathione S transferase and antigen-5, which play roles in the insect's defense and resistance against insecticide, were also observed.
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► The genus
Rhodnius (Gk rhodon, rose) comprise several species of triatomines dwelling in the Amazon. ► The LC-MS/MS proteomes of
Rhodnius sp. show proteins and transcripts; one third of which described here for the first time. ► The proteins secure blood meals, resistance against insecticide, and transmission of
Trypanosoma cruzi to humans.