Effect of Salmonella Treatment on an Implanted Tumor (CT26) in a Mouse Model

• Published in: The journal of microbiology Vol. 50; no. 3; pp. 502 - 510
• Main Authors: Yun, M.S., Chonnam National University Medical School, Gwangju, Republic of Korea, Pan, SangO, Jeollanamdo Institute of Natural Resources Research, Jangheung, Republic of Korea, Jiang, Sheng-Nan, Chonnam National University Medical School, Gwangju, Republic of Korea, Vu Hong Nguyen, Chonnam National University Medical School, Gwangju, Republic of Korea, Park, S.H., Chonnam National University Medical School, Gwangju, Republic of Korea, Jung, C.H., Chonnam National University, Gwangju, Republic of Korea, Kim, H.S., Chonnam National University Medical School, Gwangju, Republic of Korea, Min, J.J., Chonnam National University Medical School, Gwangju, Republic of Korea, Choy, H.E., Chonnam National University Medical School, Gwangju, Republic of Korea, Hong, Y.J., Chonnam National University Medical School, Gwangju, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Heidelberg The Microbiological Society of Korea 01-06-2012; Springer Nature B.V; 한국미생물학회
• Subjects: Animals; bacterial tumor targeting; Biological Therapy - methods; Biomedical and Life Sciences; Colonic Neoplasms - chemistry; Colonic Neoplasms - therapy; CT26; Disease Models, Animal; Electrophoresis, Gel, Two-Dimensional; Life Sciences; Male; Mass Spectrometry; Mice; Mice, Inbred BALB C; Microbiology; protein response; Proteins; Proteome - analysis; Salmonella; SALMONELLA TYPHIMURIUM; Salmonella typhimurium - growth & development; Salmonella typhimurium - pathogenicity; Tumors; 생물학; protein response; bacterial tumor targeting; CT26; Typhimurium
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-012-2090-9

The use of bacteria has contributed to recent advances in targeted cancer therapy especially for its tumor-specific accumulation and proliferation. In this study, we investigated the molecular events following bacterial therapy using an attenuated Salmonella Typhimurium defective in ppGpp synthesis (ΔppGpp), by analyzing those proteins differentially expressed in tumor tissues from treated and untreated mice. CT26 murine colon cancer cells were implanted in BALB/c mice and allowed to form tumors. The tumor-bearing mice were treated with the attenuated Salmonella Typhimurium. Tumor tissues were analyzed by 2D-PAGE. Fourteen differentially expressed proteins were identified by mass spectrometry. The analysis revealed that cytoskeletal components, including vimentin, drebrin-like protein, and tropomyosinalpha 3, were decreased while serum proteins related to heme or iron metabolism, including transferrin, hemopexin, and haptoglobin were increased. Subsequent studies revealed that the decrease in cytoskeletal components occurred at the transcriptional level and that the increase in heme and iron metabolism proteins occurred in liver. Most interestingly, the same pattern of increased expression of transferrin, hemopexin, and haptoglobin was observed following radiotherapy at the dosage of 14 Gy.