Evaluation of hydrophobic nanoparticulate delivery system for insulin

• Published in: Indian journal of pharmaceutical sciences Vol. 70; no. 6; pp. 721 - 726
• Main Authors: Singnurkar, P S, Gidwani, S K
• Format: Journal Article
• Language: English
• Published: India Medknow Publications and Media Pvt. Ltd 01-11-2008; Medknow Publications
• Subjects: Research Paper; nanoparticles; stearic acid; solvent diffusion; Insulin; oral delivery
• ISSN: 0250-474X; 1998-3743
• DOI: 10.4103/0250-474X.49091

Insulin loaded hydrophobic nanoparticles were prepared by solvent diffusion followed by lyophilization. Nanoparticles were characterized for mean size by dynamic laser scattering and for shape by scanning electron microscopy. Insulin encapsulation efficiency, in vitro stability of nanoparticles in presence of proteolytic enzymes and in vitro release were determined by high pressure liquid chromatography analysis. The biological activity insulin from the nanopraticles was estimated by enzyme-linked immunosorbant assay and in vivo using Wister diabetic rats. Nanoparticles ranged 0.526±0.071 μm in diameter. Insulin encapsulation efficiency was 95.7±1.2%. Insulin hydrophobic nanoparticles suppressed insulin release promoted sustained release in pH 7.4 phosphate buffer and shown to protect insulin from enzymatic degradation in vitro in presence of chymotripsin. Nanoencapsulated insulin was bioactive, demonstrated through both in vivo and in vitro.