Whole-mount Immunohistochemistry to Visualize Mouse Embryonic Dermal Lymphatic Vasculature

• Published in: Bio-protocol Vol. 11; no. 20; p. e4186
• Main Authors: Geng, Xin, Srinivasan, Sathish R
• Format: Journal Article
• Language: English
• Published: United States Bio-Protocol 20-10-2021; Bio-protocol LLC
• Subjects: Biology; Methods; Lymphatic vessels; iDISCO; Imaging; Whole-mount immunohistochemistry; Lymphatic valves
• ISSN: 2331-8325; 2331-8325
• DOI: 10.21769/BioProtoc.4186

Lymphatic vessels are abundant in the skin where they regulate interstitial fluid uptake and immune surveillance. Defects in dermal lymphatic vessels, such as fewer vessels and abnormal lymphatic vessel coverage with mural cells, are frequently associated with lymphedema and other lymphatic disorders. Whole-mount immunohistochemistry allows the visualization of dermal lymphatic vessels and identifies morphogenetic defects. Most dermal lymphatic vessels start growing during embryogenesis from lymph sacs that are located close to the axilla towards the dorsal and ventral midlines. Here, we present an approach that we have developed to permeabilize, immunolabel, clear, and visualize the lymphatic vessels. These simple and inexpensive techniques reproducibly generate images of dermal lymphatic vessels with great clarity.