DNA Damage Inducible Protein 1 is Involved in Cold Adaption of Harvested Cucumber Fruit

• Published in: Frontiers in plant science Vol. 10; p. 1723
• Main Authors: Wang, Bin, Wang, Guang, Zhu, Shijiang
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 24-01-2020
• Subjects: chilling tolerance; cold acclimation; cucumber fruit; DNA damage response; H2O2; Plant Science; cucumber fruit; chilling tolerance; cold acclimation; H2O2; DNA damage response
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2019.01723

Chilling stress can cause cellular DNA damage, affecting the faithful transmission of genetic information. Cold acclimation enhances chilling tolerance, but it is not clear that the process of cold adaption involves DNA damage responses, as cold acclimation does not form real chilling stress. Here we showed with cucumber fruit that pre-storage cold acclimation (PsCA) reduces chilling injury and upregulates DNA damage inducible protein1 (
), suggesting that the chilling tolerance induced by cold acclimation involves
transcription. Application of nitric oxide (NO), abscisic acid (ABA) or H
O
biosynthesis inhibitor before PsCA treatment downregulates
and aggravates chilling injury, while H
O
generation inhibition plus exogenous NO or ABA application before PsCA treatment restores chilling tolerance, but does not restore
expression, suggesting H
O
plays a crucial role in triggering cold adaption.
overexpression
lines show faster growth, stronger chilling tolerance, lower reactive oxygen species levels, enhanced catalase and superoxide dismutase activities and higher expression of nine other
defense genes under chilling stress, suggesting
strengthens defenses against chilling stress by enhancing antioxidant defense system. Taken together,
positively regulates chilling tolerance induced by cold acclimation in cucumber. In addition, H
O
is involved in initiation of cold acclimation. While
upregulation requires H
O
as a key signaling molecule, the upregulation of
activates an antioxidant system to reduce biotoxic accumulation of H
O
and helps in DNA repair.