Follicular Non-Hodgkin’s Lymphoma Cell Adhesion to Normal Germinal Centers and Neoplastic Follicles Involves Very Late Antigen-4 and Vascular Cell Adhesion Molecule-1

• Published in: Blood Vol. 79; no. 1; pp. 206 - 212
• Main Authors: Freedman, Arnold S., Munro, J. Michael, Morimoto, Chikao, McIntyre, Bradley W., Rhynhart, Kurt, Lee, Nina, Nadler, Lee M.
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 01-01-1992; The Americain Society of Hematology
• Subjects: Antibodies, Monoclonal; B-Lymphocytes - pathology; Biological and medical sciences; Cell Adhesion; Cell Adhesion Molecules - immunology; Cell Adhesion Molecules - physiology; Flow Cytometry; Fluorescent Antibody Technique; Hematologic and hematopoietic diseases; Humans; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymph Nodes - pathology; Lymphoma, Follicular - immunology; Lymphoma, Follicular - pathology; Medical sciences; Receptors, Very Late Antigen - immunology; Receptors, Very Late Antigen - physiology; Vascular Cell Adhesion Molecule-1; Human; Pathophysiology; Germinative center; Lymphoproliferative syndrome; Cell adhesion molecule; Follicular lymphoma; Malignant hemopathy; Non Hodgkin lymphoma; Adhesion
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V79.1.206.206

Follicular lymphomas recapitulate the architecture of germinal centers (GCs) of normal secondary lymphoid follicles. Using an in vitro binding assay, it has recently been demonstrated that the normal B lymphocytes bind to GCs. This interaction is mediated by a receptor-ligand pair consisting of the p, integrin very late antigen 4 (VLA-4) on the B cell, and the vascular cell adhesion molecule-I (VCAM-1) expressed on follicular dendritic cells (FDC). Considering the similarities between follicular lymphomas and normal GCs, the adhesive interaction of follicular non-Hodgkin's lymphoma (NHL) cells and GCs was examined. Cells isolated from 16 of 24 cases of follicular NHL bound to normal GCs. Neoplastic follicles cwld similarly support the binding of follicular NHL cells. This adhesion was inhibited by monoclonal antibodies (Mo- Abs) directed against VIA-4 and VCAM-l. This supports the hypothesis that the neoplastic follicles used the identical adhesive interactions responsible, at least in part, for the localization of normal B cells to GCs. Adhesion receptors have an important role in the regulation of normal lymphoid cell proliferation, differentiation, and localization. Therefore, an understanding of the adhesive interaction of follicular NHL cells with GCs may provide insight into the clinical and biologic behavior of these diseases.
© 1992 by The American Society of Hematology. 0006-4971/92/7901-0033$3.00/0