Separation of sulfoalkylated cyclodextrins with hydrophilic interaction liquid chromatography

• Published in: Journal of Chromatography A Vol. 1316; pp. 92 - 96
• Main Authors: Jaramillo, Michael, Kirschner, Daniel L., Dai, Zhipeng, Green, Thomas K.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 05-11-2013; Elsevier
• Subjects: Analytical chemistry; Charge; Charging; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography; Chromatography, Liquid - methods; Cyclodextrins; Cyclodextrins - analysis; Cyclodextrins - chemistry; Cyclodextrins - isolation & purification; Exact sciences and technology; Hydrophilic interaction liquid chromatography; Hydrophobic and Hydrophilic Interactions; Liquid chromatography; Models, Molecular; Molecular Weight; Other chromatographic methods; Separation; Sulfoalkylated cyclodextrins; Trace impurities; Hydrophilic interaction liquid chromatography; Sulfoalkylated cyclodextrins; Cyclodextrin; Polyanion; Chromatographic retention; Hydrophilic Interaction Liquid chromatography; chromatography; HPLC chromatography; Evaporative light scattering detector; Inclusion compound; Retention time; Hydrophilic interaction liquid; Sulfonate
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2013.09.080

•HILIC is successfully applied to analyze mixtures of sulfopropylated cyclodextrins (CDs).•Charged states of sulfopropylated CDs from −1 to −14 are baseline resolved.•Fully substituted, single isomer sulfoalkylated CDs are analyzed for purity.
Determination of the charged state distributions of partially- and fully-substituted sulfoalkylated cyclodextrins was achieved using hydrophilic interaction liquid chromatography (HILIC). HILIC analysis of a spiked sample of the partially sulfopropylated cyclodextrins was achieved using a gradient to baseline resolve the charged states from −1 to −14. The fully-substituted CDs yielded a major peak with some trace impurities and the partially-substituted sulfopropylated cyclodextrins showed a wide range of charge states present in the mixture. Small changes in the structure of the cyclodextrins have a significant impact on the retention times of the various types of cyclodextrins investigated.