Antibacterial and Toxicity Evaluation of Eastern Medicine Formulation Eczegone for the Management of Eczema

• Published in: Dose-response Vol. 18; no. 3; p. 1559325820956798
• Main Authors: Chishti, Muhammad Amjad, Mohi-Ud-Din, Ejaz, Zakki, Shahbaz Ahmad, Aslam, Muhammad Rahil, Siddiqui, Sheraz, Ahmad, Saeed, Hayee, Abdul
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01-07-2020; SAGE PUBLICATIONS, INC; SAGE Publishing
• Subjects: Albinism; Cytotoxicity; Original; Toxicity; antibacterial activity; cell viability; MTT assay; eczegone; acute oral and dermal toxicity
• ISSN: 1559-3258; 1559-3258
• DOI: 10.1177/1559325820956798

The present study was conducted to evaluate the antibacterial activity, in vitro and in vivo cytotoxicity, cell viability and safety of Eastern Medicine coded medicinal formulation Eczegone comprising extracts of Azadirachta indica (Azin), Fumaria indica (Fuin), Sphaeranthus indicus (Spin) and Lawsonia inermis (Lain). This work also evaluated antibacterial activity of Eczegone formulation having above mentioned plants ethanolic extracts against different bacteria’s by disk diffusion method. In vitro toxicity of Eczegone formulation was investigated by using human skin keratinocytes HaCaT cell line, crystal violet stained cells, and methyl tetrazolium cytotoxicity (MTT) assay. In vivo acute oral and dermal cytotoxicity was determined by using Swiss albino mice and albino rabbits, respectively. The Eczegone formulation showed antibacterial activity against 3 gram negative bacteria including Escherichia coli, Klebsiella pneumonia, Proteus vulgaris and a gram positive Staphylococcus aureus. We didn’t observe any toxic effect of Eczegone formulation on the skin keratinocytes. Furthermore, the Ezcegone formulation was non-irritant according to draize score (OECD TG404, 2002). After rigorous safety evaluation by in vitro and in vivo acute oral and dermal toxicity analysis, we concluded that Eczegone formualtion possessses antibacterial effects and is safe, non-toxic, non-irritant, and the drug would be subjected for further biochemical and clinical studies.