Development of Exon-Primed Intron-Crossing (EPIC) PCR primers for the malaria vector Anopheles pseudopunctipennis (Diptera: Culicidae)

Using the Anopheles gambiae Giles genome as a template, we designed, screened and identified 14 novel Exon-Primed Intron-Crossing (EPIC) PCR primer pairs for Anopheles pseudopunctipennis Theobald 1901, a major vector of human Plasmodium sp. in South America. These primers were designed to target the...

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Bibliographic Details
Published in:	Comptes rendus. Biologies Vol. 335; no. 6; p. 398
Main Authors:	Lardeux, Frédéric, Aliaga, Claudia, Tejerina, Rosenka, Ursic-Bedoya, Raùl
Format:	Journal Article
Language:	English
Published:	France 01-06-2012
Subjects:	Alleles Animals Anopheles - genetics Bolivia Conserved Sequence DNA Primers - genetics Exons Female Insect Vectors - genetics Introns Malaria, Falciparum - transmission Polymerase Chain Reaction - methods Polymorphism, Genetic Species Specificity Bolivia
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Summary:	Using the Anopheles gambiae Giles genome as a template, we designed, screened and identified 14 novel Exon-Primed Intron-Crossing (EPIC) PCR primer pairs for Anopheles pseudopunctipennis Theobald 1901, a major vector of human Plasmodium sp. in South America. These primers were designed to target the conserved regions flanking consecutive exons of different genes and enabled the amplification of 17 loci of which nine were polymorphic. Polymorphisms at these loci ranged from two to four alleles. Intron length polymorphism analysis is a useful tool, which will allow the study of the population structure of this mosquito species, which remains poorly understood.
ISSN:	1768-3238
DOI:	10.1016/j.crvi.2012.05.002