Electrochemical monitoring of the Pseudomonas aeruginosa growth and the formation of a biofilm in TSB media

• Published in: Bioelectrochemistry (Amsterdam, Netherlands) Vol. 150; p. 108344
• Main Authors: Pellé, J., Longo, M., Le Poul, N., Hellio, C., Rioual, S., Lescop, B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-04-2023; Elsevier
• Subjects: Bacterial Adhesion; Biofilm formation; Biofilms; Biofouling - prevention & control; Carbon; Condensed Matter; Cyclic voltammetry; Electrochemical impedance spectroscopy; Materials Science; Open-circuit potential; Physics; Pseudomonas aeruginosa; Cyclic voltammetry; Open-circuit potential; Pseudomonas aeruginosa; Biofilm formation; Electrochemical impedance spectroscopy
• ISSN: 1567-5394; 1878-562X
• DOI: 10.1016/j.bioelechem.2022.108344

•The accurate monitoring of biofilm formation is possible using OCP monitoring.•The verification of OCP results by EIS provides convincing results.•The formation process of biofilm is split into 3 steps.•An insulating layer is naturally formed in TSB culture media on GC electrode.
Understanding and sensing microbial biofilm formation onto surfaces remains highly challenging for preventing corrosion and biofouling processes. For that purpose, we have thoroughly investigated biofilm formation onto glassy carbon electrode surfaces by using electrochemical technics. Pseudomonas aeruginosa was studied because of its remarkable ability to form biofilms in many environments. The modification of the electrode-solution interface during biofilm growth was monitored by in-situ measurement of the open-circuit potential and correlated with results obtained by electrochemical impedance spectroscopy, cyclic voltammetry, scanning electron microscopy and bioassays. The sensing of the biofilm formation hence suggests a multi-steps mechanism, which may include pre-formation of an insulating layer onto the surface prior to the bacteria adhesion and biofilm formation.