Application of proteomics for comparison of proteome of Neospora caninum and Toxoplasma gondii tachyzoites

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 815; no. 1; pp. 305 - 314
• Main Authors: Lee, Eung-goo, Kim, Jae-hoon, Shin, Yong-seung, Shin, Gee-wook, Kim, Young-rim, Palaksha, K.J., Kim, Dae-yong, Yamane, Itsuro, Kim, Yong-hwan, Kim, Gon-sup, Suh, Myung-deuk, Jung, Tae-sung
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05-02-2005
• Subjects: Animals; Antigen spots; Antigens, Protozoan - analysis; Electrophoresis, Gel, Two-Dimensional; Immunoblotting; Mass Spectrometry; Neospora - chemistry; Neospora - classification; Neospora - genetics; Neospora - growth & development; Neospora caninum; Proteome - analysis; Protozoan Proteins - analysis; Protozoan Proteins - genetics; Toxoplasma - chemistry; Toxoplasma - classification; Toxoplasma - genetics; Toxoplasma - growth & development; Toxoplasma gondii; Two-dimensional gel electrophoresis; Toxoplasma gondii; Antigen spots; Mass spectrometry; Neospora caninum; Two-dimensional gel electrophoresis; Proteome analysis
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2004.08.048

Protein profiles of two isolates of
Neospora caninum (KBA-2 and JPA1) and
Toxoplasma gondii RH strain were investigated by proteomic approach. Approximately, 78% of protein spots on two-dimensional gel electrophoresis (2-DE) profiles and 80% of antigen spots on 2-DE immunoblotting profiles were exhibited to share the same p
I and
M
r between KBA-2 and JPA1 of
N. caninum. On the other hand, a total of 30 antigen spots of
T. gondii were recognized on 2-DE immunoblotting profile using rabbit antiserum against
N. caninum KBA-2. A number of homologue proteins, such as heat shock protein 70, tubulin α- and β-chain, putative protein disulfide isomerase, actin, enolase and 14-3-3 protein homologue are believed as the conserved proteins in both
N. caninum and
T. gondii. On the contrary, NcSUB1, NcGRA2 and NCDG1 (NcGRA7) might be the species-specific proteins for
N. caninum tachyzoites. The present study showed that the high degree of similarity between
N. caninum isolates (KBA-2 and JPA1), whereas large differences between
N. caninum and
T. gondii were noticed by proteome comparisons.