Active fraction of clove induces apoptosis via PI3K/Akt/mTOR-mediated autophagy in human colorectal cancer HCT-116 cells

Active fraction of clove induces apoptosis via PI3K/Akt/mTOR-mediated autophagy in human colorectal cancer HCT-116 cells

Previous studies by our group have demonstrated that extract of clove exhibits potent anticancer effects in vitro and in vivo. In the present study, the effect of an extracted and isolated active fraction of clove (AFC) on induction of cellular apoptosis in human colorectal cancer HCT-116 cells was...

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Bibliographic Details
Published in:International journal of oncology Vol. 53; no. 3; pp. 1363 - 1373
Main Authors: Liu, Minghua, Zhao, Ge, Zhang, Dan, An, Weixiao, Lai, Honglin, Li, Xiaofang, Cao, Shousong, Lin, Xiukun
Format: Journal Article
Language:English
Published: Athens Spandidos Publications 01-09-2018
Spandidos Publications UK Ltd
Subjects:
Apoptosis
Autophagy
Cancer therapies
Care and treatment
Cell culture
Cell growth
Cervical cancer
Chinese medicine
Cloves
Colorectal cancer
Cytotoxicity
Development and progression
Gene expression
Health aspects
Immunoglobulins
Kinases
Penicillin
Plant extracts
Signal transduction
Beijing China
United States > US
China
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Summary:Previous studies by our group have demonstrated that extract of clove exhibits potent anticancer effects in vitro and in vivo. In the present study, the effect of an extracted and isolated active fraction of clove (AFC) on induction of cellular apoptosis in human colorectal cancer HCT-116 cells was investigated by morphological observation, flow cytometry, and western blotting analysis. The results revealed that AFC induced apoptosis of HCT-116 cells. AFC also induced autophagy, demonstrated by increased punctuate microtubule-associated protein 1A/1B-light chain 3 (LC3) staining, and LC3-II and Beclin-1 protein expression levels. Furthermore, the autophagy inhibitors 3-MA and baflomycin A1 potentiated the pro-apoptotic activity of AFC in HCT-116 cells. AFC also inhibited the phosphorylation of the phosphoinositide 3-kinase/Akt/mechanistic target of rapamycin signaling pathway. The present study may improve the existing understanding of the anticancer mechanisms of clove and provide a scientific rationale for AFC to be further developed as a promising novel anticancer agent for the treatment of colorectal cancer. Key words: clove, apoptosis, autophagy, coloretal cancer, phosphoinositide 3-kinase/Akt/mechanistic target of rapamycin pathway
ISSN:1019-6439
1791-2423
DOI:10.3892/ijo.2018.4465