Protective effects of erdosteine on rotenone-induced oxidant injury in liver tissue

• Published in: Toxicology and industrial health Vol. 20; no. 6-10; pp. 141 - 147
• Main Authors: Terzi, Alpaslan, Iraz, Mustafa, Sahin, Semsettin, Ilhan, Atilla, Idiz, Nuri, Fadillioglu, Ersin
• Format: Journal Article
• Language: English
• Published: Thousand Oaks, CA SAGE Publications 01-09-2004; Sage Publications Ltd
• Subjects: Animals; Chemical and Drug Induced Liver Injury - metabolism; Chemical and Drug Induced Liver Injury - prevention & control; Free Radical Scavengers - pharmacology; Insecticides - toxicity; Lipid Peroxidation - drug effects; Liver - metabolism; Male; Oxidative Stress; Rats; Rats, Wistar; Rotenone - toxicity; Thioglycolates - pharmacology; Thiophenes - pharmacology; rotenone; antioxidants; liver; lipid peroxidation; erdosteine
• ISSN: 0748-2337; 1477-0393
• DOI: 10.1191/0748233704th208oa

Rotenone, an insecticide of botanical origin, causes toxicity through inhibition of complex I of the respiratory chain in mitochondria. This study was undertaken to determine whether rotenone-induced liver oxidant injury is prevented by erdosteine, a mucolytic agent showing antioxidant properties. There were four groups of Male Wistar Albino rats: group one was untreated as control; the other groups were treated with erdosteine (50 mg/kg per day, orally), rotenone (2.5 mg/mL once and 1 mL/kg per day for 60 days, i.p.) or rotenone plus erdosteine, respectively. Rotenone treatment without erdosteine increased xanthine oxidase (XO) enzyme activity and also increased lipid peroxidation in liver tissue P < 0.05). The rats treated with rotenone plus erdosteine produced a significant decrease in lipid peroxidation and XO activities in comparison with rotenone group PB / 0.05). Erdosteine treatment with rotenone led to an increase in catalase (CAT) and superoxide dismutase (SOD) activities in comparison with the rotenone group PB / 0.05). There was no significant difference in nitric oxide (NO) level between groups. There were negative correlations between CAT activity and malondialdehyde (MDA) level (r= -0.934, P <0.05) with between CAT and SOD activities (r= -0.714, P <0.05), and a positive correlation between SOD activity and MDA level (r= 0.828, P <0.05) in rotenone group. In the rotenone plus erdosteine group, there was a negative correlation between XO activity and NO level in liver tissue (r= -0.833, P -0.05). In the light of these findings, erdosteine may be a protective agent for rotenone-induced liver oxidative injury in rats.