Cooked Black Turtle Beans Ameliorate Insulin Resistance and Restore Gut Microbiota in C57BL/6J Mice on High-Fat Diets

• Published in: Foods Vol. 10; no. 8; p. 1691
• Main Authors: Tan, Yuqing, Tam, Christina C., Meng, Shi, Zhang, Yan, Alves, Priscila, Yokoyama, Wallace
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 22-07-2021; MDPI
• Subjects: Antioxidants; Bar codes; Beans; Biomarkers; c-Jun protein; Consumption; cooked black turtle bean; Cytokines; Diabetes; Diabetes mellitus; Diet; Enzymes; Food science; Glucagon; Glucose; gut microbiome; Health promotion; High fat diet; Inflammation; Insulin; Insulin resistance; Interferon; Interleukin 4; Interleukin 5; Interleukins; Intestinal microflora; LDL; Legumes; Leptin; Low density lipoprotein; Low fat diet; Microbiomes; Microbiota; Nutrient deficiency; Obesity; Plasma; Polypeptides; Signal transduction; Sterol regulatory element-binding protein; Taxonomy; Transcription factors; Triglycerides; Tumor necrosis factor-α; Turtles; Milpitas California; United States > US
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods10081691

Colored common beans are associated with health promoting and chronic disease prevention effects. Male C57BL/6J mice were fed high-fat (HF) diets supplemented with cooked black turtle beans (HFB) to prevent obesity related insulin resistance. Mice on both HF and HFB were obese compared to mice fed a low-fat (LF) diet. Plasma low density lipoprotein (LDL) and triglyceride concentrations of mice fed HFB diet were 28% and 36.6% lower than those on HF diet. Homeostatic model assessment of insulin resistance (HOMA-IR) index of mice fed HFB diet was 87% lower than that of mice fed HF diet. Diabetes related biomarkers, gastric inhibitory polypeptide (GIP), leptin, glucagon, and inflammatory cytokines interleukin 4 (IL-4) and IL-5, 10 and 12, IFN-g and TNF-α were significantly affected by HFB diet. Pparα, Cyp7a1 and Fasn were down-regulated by HFB diet while LDL-R, Srebp-2, Adipoq and Slc2a4 were up-regulated by HFB diet. The ratio of Firmicutes/Bacteroidetes (F/B) was also decreased 64.1% by HFB diet compared to HF diet. The results indicated that cooked black turtle bean consumption could ameliorate insulin resistance and lower plasma LDL in mice fed HF diet through glucose signaling pathway and JNK/c-Jun pathway. Meanwhile, cooked black turtle bean consumption restored the gut microbiome.