Evaluation of the Antifungal Activity of the Licania Rigida Leaf Ethanolic Extract against Biofilms Formed by Candida Sp. Isolates in Acrylic Resin Discs

• Published in: Antibiotics (Basel) Vol. 8; no. 4; p. 250
• Main Authors: de Freitas, Maria Audilene, Silva Alves, Adryelle Idalina, Andrade, Jacqueline Cosmo, Leite-Andrade, Melyna Chaves, Lucas Dos Santos, Antonia Thassya, Felix de Oliveira, Tatiana, Dos Santos, Franz de Assis G, Silva Buonafina, Maria Daniela, Melo Coutinho, Henrique Douglas, Alencar de Menezes, Irwin Rose, Bezerra Morais-Braga, Maria Flaviana, Pereira Neves, Rejane
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04-12-2019; MDPI
• Subjects: Acrylic resins; Antifungal activity; Antifungal agents; Antimicrobial agents; Biofilms; Candida; Candida krusei; Chromatography; Composition; Control; Dimers; Enzymes; Evaluation; Extracellular matrix; Flavonoids; Fluconazole; Fungicides; Health aspects; Herbal medicine; High performance liquid chromatography; Infections; Isoflavones; Leaves; Liquid chromatography; Magnoliopsida; Mass spectrometry; Mass spectroscopy; Medicinal plants; Microbial mats; Microorganisms; Minimum inhibitory concentration; Pathogens; Phenolic compounds; Phenols; Planktonic cells; Quadrupoles; Quercetin; Scientific imaging; Spectroscopy; Time; Brazil; Licania rigida; acrylic resin disc; Candida sp; biofilm
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics8040250

sp. treatment has become a challenge due to the formation of biofilms which favor resistance to conventional antifungals, making the search for new compounds necessary. The objective of this study was to identify the composition of the
Benth. leaf ethanolic extract and to verify its antifungal activity against
sp. and its biofilms. The composition identification was performed using the ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) technique. The antifungal activity of extract and fluconazole against planktonic cells and biofilms was verified through the minimum inhibitory concentration (MIC) following biofilm induction and quantification in acrylic resin discs by reducing tetrazolic salt, with all isolates forming biofilms within 48 h. Six constituents were identified in the extract, and the compounds identified are derivatives from phenolic compounds such as flavonoids (epi) gallocatechin Dimer, epigallocatechin and gallocatechin, Myricetin-O-hexoside, Myricitrin, and Quercetin-O-rhamnoside. The extract reduced biofilm formation in some of the strains analyzed, namely
URM5732,
INCQS40042, and
URM6352. This reduction was also observed in the treatment with fluconazole with some of the analyzed strains. The extract showed significant antifungal and anti-biofilm activities with some of the strains tested.