Changes in glutamate receptor subunit 4 expression in the deafferented olfactory bulb of zebrafish

• Published in: Brain research Vol. 1044; no. 2; pp. 251 - 261
• Main Authors: Fuller, Cynthia L., Villanueva, Ruth, Byrd, Christine A.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 24-05-2005; Amsterdam Elsevier; New York, NY
• Subjects: AMPA receptor; Animals; Biological and medical sciences; Blotting, Western; Cell Count; Danio rerio; Deafferentation; Denervation - adverse effects; Female; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - physiology; iGluR4; Immunohistochemistry - methods; Male; Neurons - metabolism; Olfactory bulb; Olfactory Bulb - cytology; Olfactory Bulb - physiology; Olfactory system and olfaction. Gustatory system and gustation; Periglomerular cells; Receptors, AMPA - metabolism; Time Factors; Tyrosine 3-Monooxygenase - metabolism; Vertebrates: nervous system and sense organs; Zebrafish; iGluR4; AMPA receptor; Periglomerular cells; Sensory systems; Zebrafish; Olfactory bulb; Deafferentation; Periglomerular cell; Central nervous system; Glutamate receptor; Subunit; Encephalon; Vertebrata; Brachydanio rerio; Olfactory pathway; Pisces
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2005.03.012

The distribution of ionotropic glutamate receptor subunit 4 (iGluR4) was examined in both normal and deafferented olfactory bulbs of adult zebrafish,
Danio rerio. With the exception of the olfactory nerve layer, there was extensive labeling with antibodies to iGluR4 in the olfactory bulbs, specifically in juxtaglomerular cell bodies and their processes. These results are consistent with previous work, which has suggested differential distribution of glutamate receptors in the vertebrate olfactory system. Analysis of bulbs following olfactory organ removal revealed a significant loss of iGluR4 immunoreactivity by 24 h post-deafferentation. At 48 h after denervation, iGluR4 labeling had returned to normal levels and was retained through 3 weeks post-surgery. Thus, afferent input plays a role in reduced labeling of this protein immediately following injury, but return of immunoreactivity can occur even without sensory innervation.