Determination of Ofloxacin in Pharmaceuticals, Human Urine and Serum Using a Potentiometric Sensor

• Published in: Electroanalysis (New York, N.Y.) Vol. 23; no. 4; pp. 1013 - 1022
• Main Authors: Pimenta, A. M., Souto, M. R. S., Catarino, R. I. L., Leal, M. F. C., Lima, J. L. F. C.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01-04-2011; WILEY‐VCH Verlag
• Subjects: Chlorides; Electrodes; Human; Ion exchangers; Ion-selective electrode; Ofloxacin; Pharmaceuticals; Potentiometry; PVC membrane; Sensors; Serum; Serums; Urine
• ISSN: 1040-0397; 1521-4109; 1521-4109
• DOI: 10.1002/elan.201000608

An ofloxacin‐selective electrode was developed for application in pharmaceutical preparations, serum and urine analysis. The electrode is based on tetrakis[3,5‐bis(trifluoromethyl)phenyl]borate as ion exchanger in a poly(vinyl chloride) membrane. The sensor displayed a Nernstian response for ofloxacin over a wide concentration range (3×10−6–1×10−2 mol L−1) with a slope of 55±1 mV per decade of concentration and a practical detection limit of 1×10−6 mol L−1. The electrode was successfully applied to the determination of ofloxacin in samples with no pretreatment steps, adequate accuracy and precision and recoveries within the intervals of 94–107 %.