Supercritical Fluid Disruption of Ralstonia eutropha for Poly(β-hydroxybutyrate) Recovery

• Published in: Biotechnology progress Vol. 19; no. 5; pp. 1519 - 1523
• Main Authors: Hejazi, Parisa, Vasheghani-Farahani, Ebrahim, Yamini, Yadollah
• Format: Journal Article
• Language: English
• Published: USA American Chemical Society 01-09-2003; American Institute of Chemical Engineers
• Subjects: Biological and medical sciences; Biotechnology; Cell Culture Techniques - methods; Cell Fractionation - methods; Chromatography, Supercritical Fluid - methods; Cupriavidus necator - metabolism; Fundamental and applied biological sciences. Psychology; Hydroxybutyrates - isolation & purification; Hydroxybutyrates - metabolism; methanol; Methanol - metabolism; Polyesters - isolation & purification; Polyesters - metabolism; Pressure; Ralstonia eutropha; Temperature; Bacteria; Butyrate(hydroxy)polymer; Ralstonia eutropha; Supercritical state; Recovery; Carbon dioxide
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1021/bp034010q

A new method for disruption of Gram‐negative bacterium Ralstonia eutrophaby supercritical CO2 for poly(β‐hydroxybutyrate) (PHB) recovery is proposed. The effects of different parameters such as exposure time, pressure, temperature, volume of methanol as a modifier, and culture history on cell disruption efficiency were investigated using Taguchiapos;s statistical approach to determine optimum conditions. The optimum conditions for cell disruption and PHB recovery were as follows: exposure time, 100 min; pressure, 200 atm; temperature, 40 °C; volume of methanol, 0.2 mL. The cell culture time was less significant. At optimum conditions, the maximum efficiency of PHB recovery was found to be 89%. The proposed method is comparable with other recovery methods in terms of the percentage of PHB recovery, while it is environmentally more benign.