Immunodiagnosis of fasciolosis using recombinant procathepsin L cystein proteinase

• Published in: Diagnostic microbiology and infectious disease Vol. 41; no. 1; pp. 43 - 49
• Main Authors: Carnevale, Silvana, Rodrı́guez, Móonica I., Guarnera, Eduardo A., Carmona, Carlos, Tanos, Tamara, Angel, Sergio O.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01-09-2001; Elsevier
• Subjects: Amino Acid Sequence; Animals; Antibodies, Helminth - biosynthesis; Antibodies, Helminth - blood; Biological and medical sciences; Cathepsin L; Cathepsins - biosynthesis; Cathepsins - chemistry; Cathepsins - immunology; Cysteine Endopeptidases - biosynthesis; Cysteine Endopeptidases - chemistry; Cysteine Endopeptidases - immunology; Cysticercosis; Diseases caused by cestodes; Enzyme Precursors - biosynthesis; Enzyme Precursors - chemistry; Enzyme Precursors - immunology; Enzyme-Linked Immunosorbent Assay; Fasciola hepatica - immunology; Fasciola hepatica - isolation & purification; Fascioliasis - diagnosis; Fascioliasis - immunology; Fascioliasis - veterinary; Fundamental and applied biological sciences. Psychology; Helminthic diseases; Humans; Immunoglobulin G - blood; Infectious diseases; Medical sciences; Microbiology; Parasitic diseases; Recombinant Fusion Proteins - immunology; Reverse Transcriptase Polymerase Chain Reaction; RNA, Helminth; Sensitivity and Specificity; Sheep; Sheep Diseases - immunology; Techniques used in virology; Virology; Escherichia coli; Trematoda; Serine-type carboxypeptidases; Bacteria; Diagnosis; Fusion protein; Ungulata; Enterobacteriaceae; Human; Serine endopeptidases; Omptin; Enzyme; Secretion; Plathelmintha; Carboxypeptidase C; Parasitosis; Infection; Antigen; Peptidases; Fasciola hepatica; Vertebrata; Sensitivity; Mammalia; Helmintha; Hydrolases; Sheep; Artiodactyla; Invertebrata; ELISA assay
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/S0732-8893(01)00288-7

Cathepsin L1, a cystein protease secreted by the gastrodermis of juvenile and adult
Fasciola hepatica, was expressed in
Escherichia coli as a fusion protein containing the proregion, supplied with six histidyl residues at the N-terminal end (rproCL1).
In this study we tested its potential as antigen for the serologic diagnosis of
F. hepatica infections by enzyme-linked aimmunosorbent assay (ELISA). The analyzed human sera included 16 positive samples, 99 negative controls and 111 from individuals affected by other parasitic and non parasitic diseases. The sensitivity and specificity of the rproCL1-ELISA were 100%. We also assessed the ability to detect antibodies in sera from 10 experimentally infected sheep, obtaining preliminary results that shown a response since the third week post infection in all the studied animals.
Therefore, the recombinant rproCL1-based ELISA could be a standardized test for the accurate diagnosis of fasciolosis.