Stretch induces a growth factor in alveolar cells via protein kinase

• Published in: Respiration physiology Vol. 127; no. 2; pp. 105 - 111
• Main Authors: Yamamoto, Hiroyuki, Teramoto, Hidemi, Uetani, Kohsaku, Igawa, Katsutoshi, Shimizu, Eiji
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 01-09-2001; Amsterdam Elsevier
• Subjects: Air breathing; Biological and medical sciences; Cell, alveolar, stretch; Cells, Cultured; Disease, inflammation; Enzyme Inhibitors - pharmacology; Enzymes, protein kinase C, alveolar cell; Epithelial Cells - cytology; Epithelial Cells - enzymology; Fundamental and applied biological sciences. Psychology; Gene Expression - physiology; Hepatocyte Growth Factor - genetics; Humans; Interleukin-8 - genetics; Mammals, humans; Mediators, growth factor, alveolar cell; Mediators, interleukin-8; Peptides - pharmacology; Protein Kinase C - antagonists & inhibitors; Protein Kinase C - metabolism; Pulmonary Alveoli - cytology; Pulmonary Edema - metabolism; Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; Signal Transduction - physiology; Stress, Mechanical; Stretch, alveolar, growth factor; Vertebrates: respiratory system; Mammals, humans; Mediators, growth factor, alveolar cell; Cell, alveolar, stretch; Disease, inflammation; Enzymes, protein kinase C, alveolar cell; Mediators, interleukin-8; Stretch, alveolar, growth factor; Human; Protein kinase C; Enzyme; Hepatocyte growth factor; Transferases; Lung; Cytokine; Gene expression; Respiratory system; In vitro; Signal transduction; Cell line; Messenger RNA; Mechanical stimulus; Stretching; Interleukin 8; Release; Growth factor; Response stimulus relation
• ISSN: 0034-5687
• DOI: 10.1016/S0034-5687(01)00244-4

Positive-pressure mechanical ventilation can injure the lung, causing edema and alveolar inflammation in a complication termed ventilator-induced lung injury (VILI). Cytokines such as interleukin-8 (IL-8) reportedly are important in this inflammatory response. On the other hand, hepatocyte growth factor (HGF) promotes regeneration of the lung, and delays pulmonary fibrosis. We postulated that cyclic stretch upregulates production and release of both of mediators. Human alveolar epithelial cells (A549) cultured on a silicoelastic membrane were tested for mRNA expression and release of IL-8 and HGF after cyclic stretch in vitro. Stretch induced mRNA expression and release of these mediators. The signaling pathway from cyclic stretch to release of IL-8 and HGF appeared to involve protein kinase C in the signal transduction pathway.