Determination of ethanol in alcoholic beverages by high-performance liquid chromatography–flame ionization detection using pure water as mobile phase

• Published in: Journal of Chromatography A Vol. 976; no. 1; pp. 387 - 391
• Main Authors: Yarita, Takashi, Nakajima, Ryoji, Otsuka, Satoko, Ihara, Toshihide, Takatsu, Akiko, Shibukawa, Masami
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 08-11-2002; Elsevier
• Subjects: Beverages - analysis; Biological and medical sciences; Brandies, spirituous liquors; Chromatography, High Pressure Liquid - methods; Ethanol; Ethanol - analysis; Fermented food industries; Flame Ionization; Food industries; Fundamental and applied biological sciences. Psychology; Water - chemistry; Wines and vinegars; Wine; Ethanol; Mobile phase composition; Detection, LC; Instrumentation; Flame ionization detection; Food analysis; Chemical analysis; White wine; Brandy; HPLC chromatography; Alcohol; Whisky; Flame ionization detector; Red wine; Sake; Quality control; Quantitative analysis
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(02)00942-1

A method for the determination of ethanol in alcoholic beverages by high-performance liquid chromatography–flame ionization detection (HPLC–FID) was developed. An FID system could be directly connected to an HPLC system using pure water as a mobile phase. In a durability test using triacontylsilyl (C
30)-silica gel stationary phase for 96 h, no significant change in the retention time of four alcohol compounds was observed. So the HPLC separation of alcoholic beverages was carried out on the C
30-silica gel stationary phase. On application to the analysis of six kinds of alcoholic beverages, ethanol could be determined accurately by the proposed method.