Hepatic DNA and RNA adduct formation from the carcinogen 7-hydroxymethyl-12-methylbenz[a]anthracene and its electrophilic sulfuric acid ester metabolite in preweanling rats and mice

• Published in: Biochemical and biophysical research communications Vol. 144; no. 2; p. 576
• Main Authors: Surh, Y J, Lai, C C, Miller, J A, Miller, E C
• Format: Journal Article
• Language: English
• Published: United States 29-04-1987
• Subjects: 9,10-Dimethyl-1,2-benzanthracene - analogs & derivatives; 9,10-Dimethyl-1,2-benzanthracene - isolation & purification; 9,10-Dimethyl-1,2-benzanthracene - metabolism; Animals; Carcinogens; Chromatography, High Pressure Liquid; DNA - metabolism; Female; Liver - metabolism; Male; Mice; Rats; RNA - metabolism; Sulfurtransferases - metabolism; Tritium
• ISSN: 0006-291X
• DOI: 10.1016/s0006-291x(87)80005-0

DNA and RNA adducts that were chromatographically identical to those formed in vitro on reaction of 7-sulfooxymethyl-12-methyl-benz[a]anthracene with guanine and adenine nucleosides were formed in the livers of rats and mice given i.p. injections of 7-hydroxymethyl- or 7-sulfooxymethyl-12-methyl-benz[a]anthracene. Considerably higher levels of these hepatic adducts were obtained from the latter short-lived electrophilic ester than from the hydroxymethyl compound. These observations are consistent with the finding of rat liver cytosolic sulfotransferase activity for 7-hydroxymethyl-12-methylbenz[a]anthracene (Watabe et al., Science 215, 403, 1982). Formation of these hepatic adducts from 7-hydroxymethyl-12-methylbenz[a]anthracene was inhibited by prior administration to rats of dehydroepiandrosterone, an inhibitor of the sulfotransferase activity for this hydroxymethyl hydrocarbon.