Optimization and validation of a capillary zone electrophoretic method for the analysis of several angiotensin-II-receptor antagonists

• Published in: Journal of Chromatography A Vol. 979; no. 1; pp. 323 - 333
• Main Authors: Hillaert, S, Van den Bossche, W
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 06-12-2002; Elsevier
• Subjects: Analysis; Angiotensin II - metabolism; Angiotensin II receptor antagonists; Angiotensin Receptor Antagonists; Biological and medical sciences; Electrophoresis, Capillary - methods; General pharmacology; Hydrogen-Ion Concentration; Medical sciences; Pharmaceutical Preparations - chemistry; Pharmacology. Drug treatments; Receptors, Angiotensin - metabolism; Reference Standards; Reproducibility of Results; Validation; Pharmaceutical analysis; Experimental design; Angiotensin II receptor antagonists; Imidazole derivatives; Tetrazole derivatives; Chemical analysis; Zone electrophoresis; Valsartan; Candesartan; Non peptide compound; Mobile phase; Optimization; Thiophene derivatives; Losartan; pH; Tablet; Eprosartan; Antagonist; Angiotensin II; Quantitative analysis; Phase composition; Capillary electrophoresis; AT1 angiotensin receptor; Telmisartan; Ultraviolet detector; Irbesartan; Benzimidazole derivatives; Biphenyl derivatives; Dosage form; Antihypertensive agent; Angiotensin antagonist; Factorial design; Chemometrics
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(02)01247-5

We optimized a capillary zone electrophoretic method for separation of six angiotensin-II-receptor antagonists (ARA-IIs): candesartan, eprosartan, irbesartan, losartan potassium, telmisartan, and valsartan. A three-level, full-factorial design was applied to study the effect of the pH and molarity of the running buffer on separation. Combination of the studied parameters permitted the separation of the six ARA-IIs, which was best carried out using 60 m
M sodium phosphate buffer (pH 2.5). The same system can also be applied for the quantitative determination of these compounds, but only for the more soluble ones. Some parameters (linearity, precision and accuracy) were validated.