Biosensor based on platinum nanoparticles dispersed in ionic liquid and laccase for determination of adrenaline

• Published in: Sensors and actuators. B, Chemical Vol. 140; no. 1; pp. 252 - 259
• Main Authors: Brondani, Daniela, Scheeren, Carla Weber, Dupont, Jairton, Vieira, Iolanda Cruz
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 18-06-2009
• Subjects: Adrenaline; Aspergillus oryzae; Biosensor; Ionic liquid; Laccase; Platinum nanoparticles; Platinum nanoparticles; Biosensor; Adrenaline; Ionic liquid; Laccase
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2009.04.037

The platinum nanoparticles dispersed in ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate (Pt-BMI.PF
6) were used in the construction of a novel biosensor for the determination of adrenaline in pharmaceutical formulations by square-wave voltammetry. This biosensor was constructed based on laccase from
Aspergillus oryzae and the responses under optimized conditions were obtained for a composition of 50:20:15:15% (w/w/w/w) graphite powder:laccase:Nujol:Pt-BMI.PF
6, phosphate buffer solution (0.1
mol
L
−1, pH 6.5), with frequency, pulse amplitude, and scan increment at 20
Hz, 80
mV, and 5.0
mV, respectively. The analytical curve was linear for adrenaline concentrations from 9.99
×
10
−7 to 2.13
×
10
−4
mol
L
−1 (
r
=
0.9998) with a detection limit of 2.93
×
10
−7
mol
L
−1. This biosensor demonstrated suitable stability (
ca. 90 days; 300 determinations) and good repeatability and reproducibility, with a relative standard deviation of 3.7% and 4.8%, respectively. The recovery study of adrenaline in pharmaceutical samples gave values from 95.5% to 104.2% and the concentrations determined are in agreement with those using the standard method at the 95% confidence level.