Identification of genes regulated by oleic acid in Jurkat cells by suppressive subtractive hybridization analysis

• Published in: FEBS letters Vol. 576; no. 3; pp. 320 - 324
• Main Authors: Martins de Lima, Thais, de Oliveira Rodrigues, Leonardo, Henrique Bengtson, Mario, Cleide Sogayar, Mari, Nogueira Alves Bezerra, Camila, Amaral Rebouças, Nancy, Curi, Rui
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 22-10-2004
• Subjects: Blotting, Northern; Cloning, Molecular; EF-1α, translation elongation factor 1 alpha; EFA, essential fatty acids; FA, fatty acid; Gene expression; Gene Expression Regulation, Neoplastic - drug effects; Humans; In Situ Hybridization - methods; Jurkat cell; Jurkat Cells; Lymphocyte; MUFA, monounsaturated fatty acid; NK, natural killer; Oleic acid; Oleic Acid - pharmacology; PCR, polymerase chain reaction; PUFA, polyunsaturated fatty acid; SFA, saturated fatty acids; SPC4, subtilisin-like protein 4; Subtractive hybridization; T-Lymphocytes; SPC4, subtilisin-like protein 4; SFA, saturated fatty acids; MUFA, monounsaturated fatty acid; Jurkat cell; Gene expression; FA, fatty acid; NK, natural killer; Oleic acid; Subtractive hybridization; EFA, essential fatty acids; PCR, polymerase chain reaction; PUFA, polyunsaturated fatty acid; EF-1α, translation elongation factor 1 alpha; Lymphocyte
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2004.09.025

In this study, the effect of oleic acid (50 μM) on gene expression of Jurkat cells (human T lymphocytes cell line) was examined using the suppressive subtractive hybridization approach. This technique allowed us to identify genes with higher or lower expression after cell treatment with oleic acid as compared to untreated cells. Oleic acid upregulated the expression of the translation elongation factor alpha 1 and ATP synthase 8 and downregulated gp96 (human tumor rejection antigen gp96), heat-shock protein 60 and subtilisin-like protein 4. These results suggest that oleic acid, at plasma physiological concentration, can regulate the expression of important genes to maintain the machinery that ensures cell functioning.