A new recombinant human bone morphogenetic protein-2 carrier for bone regeneration

• Published in: International journal of pharmaceutics Vol. 223; no. 1; pp. 69 - 79
• Main Authors: Yokota, S., Sonohara, S., Yoshida, M., Murai, M., Shimokawa, S., Fujimoto, R., Fukushima, S., Kokubo, S., Nozaki, K., Takahashi, K., Uchida, T., Yokohama, S., Sonobe, T.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 31-07-2001; Elsevier
• Subjects: Animals; Biodegradable polymer; Biological and medical sciences; Bone morphogenetic protein; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins - administration & dosage; Bone Regeneration; Bones, joints and connective tissue. Antiinflammatory agents; Drug Carriers; Gelatin; Gelatin - administration & dosage; General pharmacology; Humans; Lactic Acid - administration & dosage; Male; Medical sciences; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Polyglycolic Acid - administration & dosage; Polylactic Acid-Polyglycolic Acid Copolymer; Polymers - administration & dosage; Porifera; Porous structure; Rats; Rats, Inbred F344; Recombinant Proteins - administration & dosage; Transforming Growth Factor beta; Porous structure; Bone morphogenetic protein; Gelatin; Biodegradable polymer; Bulk density; Glycolic acid copolymer; Delivery system; Pharmaceutical technology; Implant; Rat; Control release polymer; Lactic acid copolymer; Rodentia; Drug carrier; Osteoarticular system; Vertebrata; Mammalia; Animal; Subcutaneous administration; Sponge; Coating; Bone
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/S0378-5173(01)00728-1

A gelatin sponge was formed by foaming and heat treating a gelatin solution, followed by coating the solid with poly(
d,
l-lactic-co-glycolic acid) to reinforce the gelatin framework. This sponge was tested for its suitability as a biodegradable porous, recombinant human bone morphogenetic protein (rhBMP)-2 carrier. Incorporation of rhBMP-2 into the sponge was closely related to its bulk density of gelatin sponge. The calcium content in the sponges, as assessed by an ectopic bone formation assay in rats, increased with the increasing sponge bulk density. Histologic and peripheral quantitative computed tomography analysis of implants in this ectopic assay system revealed cell growth throughout the carrier in 4 weeks after implantation regardless gelatin bulk density. The carrier containing rhBMP-2 maintained its three-dimensional structure after implantation; the carrier resisted collapse caused by soft tissue pressure during rapid bone formation as assessed by soft X-ray photographs. These results indicate that this newly developed sponge has excellent carrier characteristics to introduce rhBMP-2 into areas needed for bone regeneration.