Quantitative trait loci conferring resistance to Fusarium head blight in barley respond differentially to Fusarium graminearum infection

• Published in: Functional & integrative genomics Vol. 11; no. 1; pp. 95 - 102
• Main Authors: Jia, Haiyan, Millett, Benjamin P, Cho, Seungho, Bilgic, Hatice, Xu, Wayne W, Smith, Kevin P, Muehlbauer, Gary J
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01-03-2011; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Animal Genetics and Genomics; Barley; Biochemistry; Bioinformatics; Biological and medical sciences; Biomarkers - metabolism; Biomass; Biomedical and Life Sciences; Blight; Cell Biology; Chromosome Mapping; DNA, Plant - genetics; Fundamental and applied biological sciences. Psychology; Fusarium - genetics; Fusarium graminearum; Gene expression; Gene Expression Profiling; Gene loci; General aspects; Grain; Head; Hordeum - genetics; Hordeum - immunology; Hordeum - microbiology; Hordeum vulgare; Immunity, Innate - genetics; Infection; Life Sciences; Mathematics in biology. Statistical analysis. Models. Metrology. Data processing in biology (general aspects); Microbial Genetics and Genomics; Mycoses - genetics; Mycoses - immunology; Mycoses - microbiology; Near-isogenic line; Oligonucleotide Array Sequence Analysis; Original Paper; Plant diseases; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Genetics and Genomics; Polymerase Chain Reaction; Quantitative Trait Loci; Resistance to control; RNA profiling; scab diseases; Trichothecenes - metabolism; Triticum - genetics; Triticum aestivum; Vomitoxin; Barley; RNA profiling; Near-isogenic line; Fusarium head blight; Monocotyledones; Head; Fusarium graminearum; Hordeum vulgare; RNA; Fungi; Infection; Quantitative trait loci; Resistance; Gramineae; Angiospermae; Spermatophyta; Fungi Imperfecti; Isogenic line
• ISSN: 1438-793X; 1438-7948
• DOI: 10.1007/s10142-010-0192-1

Fusarium head blight (FHB), primarily caused by Fusarium graminearum, reduces grain yield and quality in barley. Resistance to FHB is partial and quantitatively inherited. Previously, major FHB resistant QTL were detected on barley chromosome 2H Bin 8 and 2H Bin 10, and another QTL for reduced deoxynivalenol (DON) accumulation was identified on chromosome 3H Bin 6. To develop an understanding of the molecular responses controlled by these loci, we examined DON and fungal biomass levels and the transcriptome differences in near-isogenic line (NIL) pairs carrying contrasting resistant and susceptible alleles at these QTL during F. graminearum infection. No overlap was found among the differentially accumulated transcripts of the three NIL pairs, indicating that the response to infection controlled by the resistance alleles at each QTL may be distinct. Transcripts showing differential accumulation between resistant and susceptible NILs were compared to results from previous wheat/barley-F. graminearum studies and integrated into a wheat/barley-F. graminearum interaction model.