Development of novel aptamers for low-density lipoprotein particle quantification

• Published in: PloS one Vol. 13; no. 10; p. e0205460
• Main Authors: Klapak, Daniel, Broadfoot, Sarah, Penner, Gregory, Singh, Anup, Inapuri, Eshwar
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 11-10-2018; Public Library of Science (PLoS)
• Subjects: Antisense DNA; Aptamers; Atherosclerosis; Biology and Life Sciences; Biomarkers; Biosensors; Biotechnology; Cardiovascular disease; Cardiovascular diseases; Cholesterol; Density; Deoxyribonucleic acid; Diagnostic systems; Dissociation; DNA; High density lipoprotein; Immunoglobulins; Ligands; Lipids; Low density lipoprotein; Medicine and Health Sciences; Methods; NMR; Nuclear magnetic resonance; Physical Sciences; Proteins; Research and Analysis Methods; Risk assessment; Ontario Canada; Canada; United States > US; Greenland; Pennsylvania
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0205460

Cardiovascular disease (CVD) remains the leading cause of death worldwide. Low-density lipoprotein cholesterol (LDL-C) is commonly used for CVD risk assessment; however, recent research has shown LDL particle (LDL-P) number to be a more sensitive indicator of CVD risk than both LDL-C and non-high-density lipoprotein cholesterol (HDL-C). Described herein are five single stranded DNA aptamers with dissociation constants in the low picomolar range specific to LDL-P and its subfractions. Furthermore, a set of antisense sequences have been developed and characterized that are capable of binding to the best aptamers and undergoing displacement by LDL-P for use in a simple, affordable diagnostic assay.