Reduced Expression of a Novel Midgut Trypsin Gene Involved in Protoxin Activation Correlates with Cry1Ac Resistance in a Laboratory-Selected Strain of Plutella xylostella (L.)

• Published in: Toxins Vol. 12; no. 2; p. 76
• Main Authors: Gong, Lijun, Kang, Shi, Zhou, Junlei, Sun, Dan, Guo, Le, Qin, Jianying, Zhu, Liuhong, Bai, Yang, Ye, Fan, Akami, Mazarin, Wu, Qingjun, Wang, Shaoli, Xu, Baoyun, Yang, Zhongxia, Bravo, Alejandra, Soberón, Mario, Guo, Zhaojiang, Wen, Lizhang, Zhang, Youjun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 23-01-2020; MDPI AG
• Subjects: Animals; bacillus thuringiensis; Bacillus thuringiensis Toxins - toxicity; Chymotrypsin - metabolism; cry1ac resistance; Endotoxins - toxicity; Female; Gastrointestinal Tract; Hemolysin Proteins - toxicity; Insect Proteins - genetics; Insecticide Resistance - genetics; Insecticides - toxicity; Larva - drug effects; Larva - genetics; Male; Moths - drug effects; Moths - genetics; Pest Control, Biological; Phylogeny; plutella xylostella; protoxin activation; Trypsin - genetics; Trypsin - metabolism; trypsin-like midgut protease; Cry1Ac resistance; Plutella xylostella; Bacillus thuringiensis; trypsin-like midgut protease; protoxin activation
• ISSN: 2072-6651; 2072-6651
• DOI: 10.3390/TOXINS12020076

(Bt) produce diverse insecticidal proteins to kill insect pests. Nevertheless, evolution of resistance to Bt toxins hampers the sustainable use of this technology. Previously, we identified down-regulation of a trypsin-like serine protease gene
in the midgut transcriptome and RNA-Seq data of a laboratory-selected Cry1Ac-resistant
strain, SZ-R. We show here that reduced PxTryp_SPc1 expression significantly reduced caseinolytic and trypsin protease activities affecting Cry1Ac protoxin activation, thereby conferring higher resistance to Cry1Ac protoxin than activated toxin in SZ-R strain. Herein, the full-length cDNA sequence of
gene was cloned, and we found that it was mainly expressed in midgut tissue in all larval instars. Subsequently, we confirmed that the
gene was significantly decreased in SZ-R larval midgut and was further reduced when selected with high dose of Cry1Ac protoxin. Moreover, down-regulation of the
gene was genetically linked to resistance to Cry1Ac in the SZ-R strain. Finally, RNAi-mediated silencing of
gene expression decreased larval susceptibility to Cry1Ac protoxin in the susceptible DBM1Ac-S strain, supporting that low expression of
gene is involved in Cry1Ac resistance in
. These findings contribute to understanding the role of midgut proteases in the mechanisms underlying insect resistance to Bt toxins.