Meta-CF3-Substituted Analogues of the GFP Chromophore with Remarkable Solvatochromism

In this work, we have shown that the introduction of a trifluoromethyl group into the me-ta-position of arylidene imidazolones (GFP chromophore core) leads to a dramatic increase in their fluorescence in nonpolar and aprotic media. The presence of a pronounced solvent-dependent gradation of fluoresc...

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Bibliographic Details
Published in:	International journal of molecular sciences Vol. 24; no. 12; p. 9923
Main Authors:	Perfilov, Maxim, Zaitseva, Elvira, Baleeva, Nadezhda, Kublitski, Vadim, Smirnov, Alexander, Bogdanova, Yulia, Krasnova, Svetlana, Myasnyanko, Ivan, Mishin, Alexander, Baranov, Mikhail
Format:	Journal Article
Language:	English
Published:	Basel MDPI AG 08-06-2023 MDPI
Subjects:	Chromophores Endoplasmic reticulum Fluorescence fluorogen GFP Heterocyclic compounds Kaede Labeling Localization Optical properties Proteins Solvents
Online Access:	Get full text
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Summary:	In this work, we have shown that the introduction of a trifluoromethyl group into the me-ta-position of arylidene imidazolones (GFP chromophore core) leads to a dramatic increase in their fluorescence in nonpolar and aprotic media. The presence of a pronounced solvent-dependent gradation of fluorescence intensity makes it possible to use these substances as fluorescent polarity sensors. In particular, we showed that one of the created compounds could be used for selective labeling of the endoplasmic reticulum of living cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1422-0067 1661-6596 1422-0067
DOI:	10.3390/ijms24129923