Biomonitoring of urine mutagenicity with the Ames test: improvement of the extraction/concentration method

• Published in: Mutation research Vol. 520; no. 1; pp. 199 - 205
• Main Authors: Andre, Véronique, Lebailly, Pierre, Deslandes, Edwige, Henry-Amar, Michel, Gauduchon, Pascal
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 26-09-2002; Elsevier
• Subjects: Ames test; Antineoplastic Agents; Antineoplastic Agents - urine; Biological and medical sciences; Biomonitoring; Chemical mutagenesis; Chromatography, Ion Exchange; Extraction method; Female; Humans; Life Sciences; Male; Medical sciences; Mutagenicity Tests; Mutagens; Mutagens - analysis; Polystyrenes; Polystyrenes - chemistry; Salmonella typhimurium; Salmonella typhimurium - drug effects; Smoking; Smoking - urine; Toxicology; Urine mutagenicity; Biomonitoring; Ames test; Urine mutagenicity; Extraction method; Urine; Improvement; Extraction; Concentration; Method
• ISSN: 1383-5718; 0027-5107; 1879-3592
• DOI: 10.1016/S1383-5718(02)00172-9

Comparative extraction efficiency of the pre-packed Bakerbond
®-spe-SDB-1 resin and of Amberlite
®-AD2 (XAD-2) resin, for the preparation of urine extracts in biomonitoring studies. Urine extracts were prepared in parallel with the Bakerbond
® column and with the classical XAD-2 resin from urines (1) spiked with mutagenic chemicals, (2) collected from patients after chemotherapy, and (3) from smokers. Mutagenic activities were evaluated on
Salmonella typhimurium tester strains TA97a, TA98, TA100 and TA102 with and without S9 mix. Mutagenic activities obtained with Bakerbond
® extracts were almost always higher or at least equivalent to those prepared on XAD-2 resin. Similar results were observed for the three urine sample groups. When fully validated, the use of the pre-packed columns will be more convenient and time-saving for large population studies.