Discrimination between ribonuclease H- and ribonuclease L-mediated RNA degradation by 2′- O-methylated 2-5A-antisense oligonucleotides

• Published in: Bioorganic & medicinal chemistry letters Vol. 9; no. 7; pp. 1049 - 1054
• Main Authors: Cramer, Hagen, Player, Mark R., Torrence, Paul F.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 05-04-1999; Elsevier
• Subjects: Adenine Nucleotides - genetics; Analytical, structural and metabolic biochemistry; Base Sequence; Biological and medical sciences; Endoribonucleases - metabolism; Fundamental and applied biological sciences. Psychology; Hydrolysis; Methylation; Nucleic acids; Oligonucleotides, Antisense - chemistry; Oligoribonucleotides - genetics; Ribonuclease H - metabolism; RNA - metabolism; Rna, ribonucleoproteins; Spacer arm; Enzyme; Enzymatic digestion; Hydrolases; Esterases; Calf thymus ribonuclease H; Antisense DNA; Ribonuclease; Oligodeoxyribonucleotide; Antisense oligonucleotide
• ISSN: 0960-894X; 1464-3405
• DOI: 10.1016/S0960-894X(99)00134-1

2′,5′-Oligoadenylate (2-5A) antisense chimeric oligonucleotides were synthesized containing varying 2′-
O-methyl-ribonucleotide substitution patterns in the antisense domain. The ability of these composite oligonucleotides to mediate RNase H- and RNase L-catalyzed RNA degradation showed that these two enzymes have different activation requirements.
2′,5′-Oligoadenylate antisense oligonucleotides varying 2′-O-methyl-ribonucleotide substitution patterns in the antisense domain were synthesized and evaluated for their ability to mediate RNase H- and RNase L-catalyzed RNA degradation.