Chemical sympathectomy impairs bone resorption in rats: a role for the sympathetic system on bone metabolism

• Published in: Bone (New York, N.Y.) Vol. 25; no. 5; pp. 545 - 551
• Main Authors: Cherruau, M, Facchinetti, P, Baroukh, B, Saffar, J.L
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01-11-1999; Elsevier Science
• Subjects: Alkaline Phosphatase - analysis; Alkaline Phosphatase - metabolism; Animals; Biological and medical sciences; Bone Resorption - metabolism; Bone Resorption - physiopathology; Calcitonin Gene-Related Peptide - metabolism; Calcitonin Gene-Related Peptide - physiology; CGRP; Dental Pulp - innervation; Fundamental and applied biological sciences. Psychology; Guanethidine; Male; Mandible - enzymology; Mandible - innervation; Mandible - metabolism; Nervous system; Osteoclast-osteoblast interactions; Osteoclasts - cytology; Periosteum - cytology; Periosteum - enzymology; Periosteum - innervation; Periosteum - metabolism; Preosteoclast; Rats; Rats, Wistar; Resorption; Skeleton and joints; Substance P; Substance P - metabolism; Substance P - physiology; Sympathectomy; Sympathectomy, Chemical; Sympathetic Nervous System - physiology; Vertebrates: osteoarticular system, musculoskeletal system; Sympathectomy; Substance P; Osteoclast-osteoblast interactions; Resorption; Guanethidine; Preosteoclast; Nervous system; CGRP; Animal model; Rat; Rodentia; Osteoclast; Sympathetic nervous system; In vitro; Osteoarticular system; Vertebrata; Mammalia; Neuromediator; Chemical sympathectomy; Osteoblast; Biological effect; Bone
• ISSN: 8756-3282; 1873-2763
• DOI: 10.1016/S8756-3282(99)00211-2

The possibility that the nervous system may control bone metabolism has been raised, as neuromediators physiologically conveyed by sympathetic fibers (eg, vasoactive intestinal peptide) influence bone resorption in vitro. In this study, the sympathetic system was inactivated by treating rats with guanethidine (40 mg/kg/day), a sympathetic neurotoxic, for 21 days, after which a wave of osteoclastic resorption was induced along the mandibular buccal cortex. The effects of denervation were assessed 4 days later (corresponding to the peak of resorption in this model). The rats exhibited ptosis soon after starting guanethidine, proving the success of the sympathectomy. This was associated with a significant increase in calcitonin gene-related peptide- (+54%,
p < 0.02) and substance P-immunoreactive sensory fibers (+29%,
p < 0.02), a known effect of sympathectomy. For the quantitation of the bone parameters, the study zone was divided into a juxta-osseous alkaline phosphatase-positive osteogenic compartment and a nonosteogenic compartment. In the osteogenic compartment, the resorption surface was reduced by 56% (
p < 0.001) in the treated animals, together with a fall in the number of osteoclasts (−25%,
p < 0.05) and impaired osteoclast access to the bone surface. Tartrate-resistant acid phosphatase-positive (TRAP
+) mononuclear preosteoclasts were found only in this compartment; they were reduced by 43% (
p < 0.05) by the sympathectomy. No change in nonspecific esterase (NSE)
+ osteoclast precursors was found. In the nonosteogenic compartment, vasodilation was the only effect of sympathectomy (+80%,
p < 0.05); in particular, the number of NSE
+ cells was not modified. Our results indicate that: (1) interactions of NSE
+ precursors with osteogenic cells are required for their differentiation into TRAP
+ preosteoclasts; (2) the sympathetic nervous system is not involved in osteoclast precursor recruitment; but (3) has a significant effect on resorption by inhibiting preosteoclast differentiation and disturbing osteoclast activation. These data suggest that depletion of sympathetic mediators may disturb osteogenic cell-mediated osteoclast differentiation.