New cytokine dressings. I. Kinetics of the in vitro rhG-CSF, rhGM-CSF, and rhEGF release from the dressings

• Published in: International journal of pharmaceutics Vol. 184; no. 2; pp. 173 - 178
• Main Authors: Grzybowski, Jacek, Ołdak, Ewa, Antos-Bielska, Małgorzata, Janiak, Marek K, Pojda, Zygmunt
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 20-07-1999; Elsevier
• Subjects: Bandages; Biological and medical sciences; Collagen; Cytokine dressings; Cytokines - administration & dosage; Cytokines - chemistry; Delivery systems; Drug Delivery Systems; Epidermal Growth Factor - administration & dosage; Epidermal Growth Factor - chemistry; Freeze Drying; General pharmacology; Granulocyte Colony-Stimulating Factor - administration & dosage; Granulocyte Colony-Stimulating Factor - chemistry; Granulocyte-Macrophage Colony-Stimulating Factor - administration & dosage; Granulocyte-Macrophage Colony-Stimulating Factor - chemistry; Humans; Immunomodulators; Kinetics; Medical sciences; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Polyurethanes; Recombinant Proteins - administration & dosage; Recombinant Proteins - chemistry; rh-GM-CSF; rhEGF; rhG-CSF; rh-GM-CSF; rhEGF; Cytokine dressings; rhG-CSF; Delivery systems; Delivery system; Freeze drying; Pharmaceutical technology; Cytokine; In vitro; Dressing; Granulocyte colony stimulating factor; Epidermal growth factor; Collagen; Polyurethane; Dosage form; Active ingredient; Recombinant protein; Granulocyte macrophage colony stimulating factor; Release
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/S0378-5173(99)00065-4

Wound repair-stimulatory activities of various cytokines and growth factors depend on successful delivery of these factors to the injured sites. Here were present the design and preparation of the new collagen- and polyurethane-based dressings containing the recombinant human cytokines-rhG-CSF, rhGM-CSF or rhEGF. To test the efficacy of the retrieval of the incorporated cytokines, their controlled release from the dressings was carried out over three consecutive days using polyurethane sponge as a collector of the extracts. The maximum quantities of the released rhG-CSF, rhGM-CSF and rhEGF reached approximately 25, 50, and 10%, respectively, of the total cytokine contents of the dressings, as assessed by the specific ELISA tests. These data indicate that collagen- and polyurethane dressings containing rhGM-CSF and rhG/CSF may serve as effective tools for the topical delivery of cytokines to wounded tissues.